Evaluation of early abnormalities of the sensory retina in a hypercholesterolemia experimental model: an immunohistochemical study

Evaluation of early abnormalities of the sensory retina in a hypercholesterolemia experimental model: an immunohistochemical study

Alternative title Avaliação das anormalidades precoces da retina sensorial em modelo experimental de hipercolesterolemia: estudo imunohistoquímico
Author Torres, Rogil José de Almeida Autor UNIFESP Google Scholar
Maia, Maurício Autor UNIFESP Google Scholar
Précoma, Dalton Bertolin Google Scholar
Noronha, Lucia Google Scholar
Luchini, Andréa Google Scholar
Précoma, Leonardo Brandão Google Scholar
Souza, Greyce Kelly de Google Scholar
Muccioli, Cristina Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hospital de Olhos Oeste Paulista Vitreoretinal Surgery Service
Pontifícia Universidade Católica do Paraná - PUCPR Cardiology Department
PUCPR Pathology Department
Centro Oftalmológico de Curitiba
PUCPR
Abstract PURPOSE: The aim of this study is to demonstrate the early changes of the sensory retina induced by hypercholesterolemia in an experimental model. METHODS: New Zealand rabbits were divided into two groups: CG (Control Group) was fed a normal diet for 6 weeks. G1 was initially fed a 1% cholesterol diet for two weeks and from the 14th day on a 0.5% cholesterol diet until the 42nd day. The eyes underwent an immunohistochemical analysis with monoclonal antibodies anti-calretinin and anti-glial fibrillary acidic protein (GFAP). RESULTS: G1 cells and cell elements presented significant immunoreactivity to anti-calretinin. No immunoreactivity to anti-glial fibrillary acidic protein was observed in both groups. CONCLUSION: This study has shown that a hypercholesterolemic diet may induce early changes in the sensory retina in rabbits. The anti-calretinin monoclonal antibody was able to reveal calcium accumulation inside the nerve cells.

OBJETIVO: O objetivo deste estudo é demonstrar experimentalmente as alterações precoces da retina sensorial induzidas pela hipercolesterolemia. MÉTODOS: Coelhos New Zealand foram organizados em dois grupos: GC (grupo controle), composto por 6 coelhos (6 olhos), recebeu dieta normal por 6 semanas; G1, composto por 12 coelhos (12 olhos), tratado previamente com ração colesterol a 1% (Sigma-Aldrich) por 2 semanas e a partir do 14º dia com ração colesterol a 0,5% (Sigma-Aldrich). Os olhos foram submetidos à análise imunohistoquímica com os anticorpos monoclonais anticalretinina e anti-glial fibrillary acidic protein (GFAP). RESULTADOS: G1 apresentou maior número de células e elementos celulares imunoreativos a anticalretinina que o GC, com relevância estatística. GFAP foi negativo em ambos os grupos. CONCLUSÃO: Este estudo demonstrou que a dieta hipercolesterolêmica pode induzir alterações precoces na retina sensorial em coelhos. O anticorpo monoclonal anticalretinina foi capaz de revelar o acúmulo de cálcio dentro das células neuronais retiniana.
Keywords Retina
Models, animal
Calcium-binding proteins
Cholesterol
Anoxia
Immunohistochemistry
Rabbits
Retina
Modelos animais
Proteínas de ligação do cálcio
Colesterol
Anoxia
Imuno-histoquímica
Coelhos
Language English
Date 2009-12-01
Published in Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 72, n. 6, p. 793-798, 2009.
ISSN 0004-2749 (Sherpa/Romeo, impact factor)
Publisher Conselho Brasileiro de Oftalmologia
Extent 793-798
Origin http://dx.doi.org/10.1590/S0004-27492009000600010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000274591700010
SciELO ID S0004-27492009000600010 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/5443

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