The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Autor Santos, Marcia Regina Machado Autor UNIFESP Google Scholar
Cano, Maria I Autor UNIFESP Google Scholar
Schijman, Alejandro Google Scholar
Lorenzi, Hernan Google Scholar
Vázquez, Martin Google Scholar
Levin, Mariano J Google Scholar
Ramirez, José Luis Google Scholar
Brandão, Adeílton Google Scholar
Degrave, Wim M Google Scholar
Franco da Silveira, José Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular
Universidad Central de Venezuela
Instituto Oswaldo Cruz
Resumo By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes
Palavra-chave Trypanosoma cruzi
clone CL
Brener
genome project
molecular karyotype
chromosome mapping
genetic map
Idioma Inglês
Data de publicação 1997-11-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.
ISSN 0074-0276 (Sherpa/Romeo)
Publicador Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 821-828
Fonte http://dx.doi.org/10.1590/S0074-02761997000600018
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S0074-02761997000600018 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/544

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