Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener

Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener

Author Ferrari, I Google Scholar
Lorenzi, H Google Scholar
Santos, Mr Autor UNIFESP Google Scholar
Brandariz, S Google Scholar
Requena, Jm Google Scholar
Schijman, A Google Scholar
Vázquez, M Google Scholar
Franco da Silveira, José Autor UNIFESP Google Scholar
Ben-dov, C Google Scholar
Medrano, C Google Scholar
Ghío, S Google Scholar
López Bergami, P Google Scholar
Cano, I Autor UNIFESP Google Scholar
Zingales, B Google Scholar
Urmenyi, Tp Google Scholar
Rondinelli, E Google Scholar
González, A Google Scholar
Cortes, A Google Scholar
Lopez, Mc Google Scholar
Thomas, Mc Google Scholar
Alonso, C Google Scholar
Ramírez, Jl Google Scholar
Chiurrillo, Ma Google Scholar
Aldao, R Rangel Google Scholar
Brandão, A Google Scholar
Degrave, W Google Scholar
Perrot, V Google Scholar
Saumier, M Google Scholar
Billaut, A Google Scholar
Cohen, D Google Scholar
Le Paslier, D Google Scholar
Levin, Mj Google Scholar
Institution Instituto de Investigaciones en Ingeniería Genética y Biología Molecular
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Federal do Rio de Janeiro
Universidad Central de Venezuela
Instituto Oswaldo Cruz
Abstract Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease
Keywords Trypanosoma cruzi
genome project
physical map
Yac library
BAC library
sequence tagged sites (STS)
expressed sequence tags (EST)
Language English
Date 1997-11-01
Published in Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 843-852, 1997.
ISSN 0074-0276 (Sherpa/Romeo)
Publisher Instituto Oswaldo Cruz, Ministério da Saúde
Extent 843-852
Access rights Open access Open Access
Type Article
SciELO ID S0074-02761997000600021 (statistics in SciELO)

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