Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?

Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?

Author de Morais-Zani, Karen Google Scholar
Serino-Silva, Caroline Google Scholar
Galizio, Nathalia da Costa Google Scholar
Tasima, Lidia Jorge Google Scholar
Pagotto, Josias Falararo Autor UNIFESP Google Scholar
Teixeira da Rocha, Marisa Maria Google Scholar
Marcelino, Jose Roberto Google Scholar
Sant'Anna, Savio Stefanini Google Scholar
Tashima, Alexandre Keiji Autor UNIFESP Google Scholar
Tanaka-Azevedo, Anita Mitico Google Scholar
Grego, Kathleen Fernandes Google Scholar
Abstract Considering that the scarcity of venom represents a huge challenge for biochemical and functional studies of Micrurus species (coral snakes), in this report we describe for the first time the influence of pilocarpine administration prior to venom milking on the yield and protein composition of Micrurus corallinus venom. The administration of pilocarpine resulted in an increase of about 127% in the volume of venom milked, with similar protein content. Venoms showed similar protein bands distribution and intensity by SDS-PAGE and equivalents RP-HPLC profiles. Our proteomic analysis showed that venoms milked in the presence and absence of pilocarpine presented comparable protein profiles, in terms of protein composition and relative abundance. The toxins identified were assigned to 13 protein families and represent the most complete M. corallinus venom proteome described so far, in terms of number of protein families identified. Our data indicate that the administration of pilocarpine prior to venom milking increases the venom yield and does not change significantly the venom composition of M. corallinus. The employment of pilocarpine represents a useful approach to increase the yield of venom not only for Micrurus species, but also for other genera of snakes with limitations regarding the amount of venom available.
Keywords Micrurus corallinus
Pilocarpine
Venom yield
Venom composition
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Fundacao de Amparo Pesquisa do Estado de Sao Paulo
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Financiadora de Estudos e Projetos (FINEP)
Grant number FAPESP: [2014/11108-0, 2016/09976-9, 2017/01890-0, 2012/19321-9, 2016/03839-0
Date 2018
Published in Journal Of Proteomics. Amsterdam, v. 174, p. 17-27, 2018.
ISSN 1874-3919 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 17-27
Origin http://dx.doi.org/10.1016/j.jprot.2017.12.010
Access rights Closed access
Type Article
Web of Science ID WOS:000425567700003
URI https://repositorio.unifesp.br/handle/11600/54080

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