Comparative proteomic analysis of Xanthomonas citri ssp citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction

Comparative proteomic analysis of Xanthomonas citri ssp citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction

Author Artier, Juliana Google Scholar
Zandonadi, Flavia da Silva Google Scholar
de Souza Carvalho, Flavia Maria Google Scholar
Pauletti, Bianca Alves Google Scholar
Paes Leme, Adriana Franco Google Scholar
Carnielli, Carolina Moretto Google Scholar
Selistre-de-Araujo, Heloisa Sobreiro Google Scholar
Bertolini, Maria Celia Google Scholar
Ferro, Jesus Aparecido Google Scholar
Belasque Junior, Jose Google Scholar
Franco de Oliveira, Julio Cezar Autor UNIFESP Google Scholar
Marques Novo-Mansur, Maria Teresa Google Scholar
Abstract Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm-enriched fraction was performed for XAC cells grown in pathogenicity-inducing (XAM-M) and pathogenicity-non-inducing (nutrient broth) media using two-dimensional electrophoresis combined with liquid chromatography-tandem mass spectrometry. Amongst the 40 proteins identified, transglycosylase was detected in a highly abundant spot in XAC cells grown under inducing condition. Additional up-regulated proteins related to cellular envelope metabolism included glucose-1-phosphate thymidylyltransferase, dTDP-4-dehydrorhamnose-3,5-epimerase and peptidyl-prolyl cis-trans-isomerase. Phosphoglucomutase and superoxide dismutase proteins, known to be involved in pathogenicity in other Xanthomonas species or organisms, were also detected. Western blot and quantitative real-time polymerase chain reaction analyses for transglycosylase and superoxide dismutase confirmed that these proteins were up-regulated under inducing condition, consistent with the proteomic results. Multiple spots for the 60-kDa chaperonin and glyceraldehyde-3-phosphate dehydrogenase were identified, suggesting the presence of post-translational modifications. We propose that substantial alterations in cellular envelope metabolism occur during the XAC infectious process, which are related to several aspects, from defence against reactive oxygen species to exopolysaccharide synthesis. Our results provide new candidates for virulence-related proteins, whose abundance correlates with the induction of pathogenicity and virulence genes, such as hrpD6, hrpG, hrpB7, hpa1 and hrpX. The results present new potential targets against XAC to be investigated in further functional studies.
Keywords cell envelope metabolism
citrus canker
differential proteomics
pathogenicity
periplasmic proteins
Xanthomonas citri ssp citri
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP, Brazil
Fundecitrus (Fundo de Defesa da Citricultura - Araraquara, SP, Brazil)
CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
Grant number FAPESP: 2007/50910-2
Date 2018
Published in Molecular Plant Pathology. Hoboken, v. 19, n. 1, p. 143-157, 2018.
ISSN 1464-6722 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 143-157
Origin http://dx.doi.org/10.1111/mpp.12507
Access rights Closed access
Type Article
Web of Science ID WOS:000417763700012
URI https://repositorio.unifesp.br/handle/11600/53966

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