Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases

Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases

Author Ramos, Gracielle Vieira Google Scholar
de Toledo-Arruda, Alessandra Choqueta Autor UNIFESP Google Scholar
Pinheiro-Dardis, Clara Maria Google Scholar
Suehiro, Camila Liyoko Google Scholar
de Russo, Thiago Luiz Google Scholar
Vieira, Rodolfo Paula Autor UNIFESP Google Scholar
Martins, Milton Arruda Google Scholar
Salvini, Tania Fatima Google Scholar
Quagliotti Durigan, Joao Luiz Google Scholar
Abstract Background. The present study aimed to analyze the effects of physical training on an antioxidant canonical pathway and metalloproteinases activity in diaphragm muscle in a model of cigarette smoke-induced chronic obstructive pulmonary disease (COPD). Methods. Male mice were randomized into control, smoke, exercise, and exercise + smoke groups, which were maintained in trial period of 24 weeks. Gene expression of kelch-like ECH-associated protein 1

nuclear factor erythroid-2 like 2

and heme-oxygenase1 by polymerase chain reaction was performed. Metalloproteinases 2 and 9 activities were analyzed by zymography. Exercise capacity was evaluated by treadmill exercise test before and after the protocol. Results. Aerobic training inhibited diaphragm muscle wasting induced by cigarette smoke exposure. This inhibition was associated with improved aerobic capacity in those animals that were submitted to 24 weeks of aerobic training, when compared to the control and smoke groups, which were not submitted to training. The aerobic training also downregulated the increase of matrix metalloproteinases (MMP-2 and MMP-9) and upregulated antioxidant genes, such as nuclear factor erythroid-2 like 2 (NRF2) and heme-oxygenase1 (HMOX1), in exercise + smoke group compared to smoke group. Conclusions. Treadmill aerobic training protects diaphragm muscle wasting induced by cigarette smoke exposure involving upregulation of antioxidant genes and downregulation of matrix metalloproteinases.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Sao Paulo Research Foundation (FAPESP) Grant [2012/15165-2]
Grant number FAPESP [2012/15165-2]
Date 2018
Published in Biomed Research International. London, v. , p. -, 2018.
ISSN 2314-6133 (Sherpa/Romeo, impact factor)
Publisher Hindawi Ltd
Extent -
Origin http://dx.doi.org/10.1155/2018/5909053
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000428593700001
URI https://repositorio.unifesp.br/handle/11600/53796

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