Identification and characterization of a rich population of CD34(+) mesenchymal stem/stromal cells in human parotid, sublingual and submandibular glands

Identification and characterization of a rich population of CD34(+) mesenchymal stem/stromal cells in human parotid, sublingual and submandibular glands

Author Togarrati, Padma Priya Google Scholar
Sasaki, Robson T. Autor UNIFESP Google Scholar
Abdel-Mohsen, Mohamed Google Scholar
Dinglasan, Nuntana Google Scholar
Deng, Xutao Google Scholar
Desai, Shivani Google Scholar
Emmerson, Elaine Google Scholar
Yee, Elizabeth Google Scholar
Ryan, William R. Google Scholar
da Silva, Marcelo C. P. Autor UNIFESP Google Scholar
Knox, Sarah M. Google Scholar
Pillai, Satish K. Google Scholar
Muench, Marcus O. Google Scholar
Abstract Mesenchymal stem/stromal cells (MSCs) play crucial roles in maintaining tissue homeostasis during physiological turnovers and injuries. Very little is known about the phenotype, distribution and molecular nature of MSCs in freshly isolated human salivary glands (SGs) as most reports have focused on the analysis of cultured MSCs. Our results demonstrate that the cell adhesion molecule CD34 was widely expressed by the MSCs of human major SGs, namely parotid (PAG), sublingual (SLG) and submandibular (SMG) glands. Further, gene expression analysis of CD34(+) cells derived from fetal SMGs showed significant upregulation of genes involved in cellular adhesion, proliferation, branching, extracellular matrix remodeling and organ development. Moreover, CD34(+) SMG cells exhibited elevated expression of genes encoding extracellular matrix, basement membrane proteins, and members of ERK, FGF and PDGF signaling pathways, which play key roles in glandular development, branching and homeostasis. In vitro CD34(+) cell derived SG-MSCs revealed multilineage differentiation potential. Intraglandular transplantation of cultured MSCs in immunodeficient mice led to their engraftment in the injected and uninjected contralateral and ipsilateral glands. Engrafted cells could be localized to the stroma surrounding acini and ducts. In summary, our data show that CD34(+) derived SG-MSCs could be a promising cell source for adoptive cell-based SG therapies, and bioengineering of artificial SGs.
xmlui.dri2xhtml.METS-1.0.item-coverage London
Language English
Sponsor National Institutes of Health
RIVA foundation
CAPES foundation
California Institute of Regenerative Medicine
Grant number National Institutes of Health: RO1 DE024188
National Institutes of Health: PO1 DK088760
National Institutes of Health: RO1 GM117901
RIVA foundation
CAPES
California Institute of Regenerative Medicine: TB1-01188
Date 2017
Published in Scientific Reports. London, v. 7, p. -, 2017.
ISSN 2045-2322 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent -
Origin http://dx.doi.org/10.1038/s41598-017-03681-1
Access rights ACESSO ABERTO
Type Article
Web of Science ID WOS:000403318400038
URI https://repositorio.unifesp.br/handle/11600/53658

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