Frequency of Toxoplasma gondii in the retina in eye banks in Brazil

Frequency of Toxoplasma gondii in the retina in eye banks in Brazil

Author Costa, Deise F. Autor UNIFESP Google Scholar
Nascimento, Heloisa Autor UNIFESP Google Scholar
Sutili, Aline Autor UNIFESP Google Scholar
Nobrega, Fernando A. J. Google Scholar
Fowler, Flavio Autor UNIFESP Google Scholar
Nobrega, Mario Junqueira Google Scholar
Garrido, Cristina Google Scholar
Dias, Janaina de Oliveira Google Scholar
Adan, Consuelo B. D. Google Scholar
Rizzo, Luiz Vicente Google Scholar
Silveira, Claudio Google Scholar
Belfort, Rubens, Jr. Autor UNIFESP Google Scholar
Commodaro, Alessandra G. Autor UNIFESP Google Scholar
Abstract Ocular toxoplasmosis is the main cause of posterior uveitis worldwide frequently leading to vision loss. In Brazil, the seroprevalence of Toxoplasma gondii infection ranges from 50 to 80% depending of the region studied. The frequency of toxoplasmic retinal scar may reach 18% of the adults in the South of Brazil. Our goal was to determine the frequency of T. gondii DNA in retinas from eye banks from different regions in Brazil. A total of 162 eyes were obtained from eye banks in Manaus (n = 60), Sao Paulo (n = 60), Chapeco (n = 26), and Joinville (n = 16). The retinas were macroscopically analyzed and collected for DNA extraction. Real-time PCR (qPCR) was performed using the T. gondii B1 marker. By qPCR, a higher frequency of T. gondii DNA in the retinas from the eye bank of Joinville (25%) was found when compared to Manaus (5%). The retinas from Sao Paulo and Chapeco were qPCR negative. Clinical examination determined the retina lesions to be compatible with toxoplasmosis in the following frequencies: Joinville (62.5%), Manaus (10%), Sao Paulo (6.7%), and Chapeco (15.4%).
Keywords Eye bank
Brazil
Toxoplasma gondii
Real-time PCR
xmlui.dri2xhtml.METS-1.0.item-coverage New York
Language English
Sponsor CNPq
FAPESP
CAPES
Grant number CNPq: 400040/2014-0
FAPESP
CAPES
Date 2017
Published in Parasitology Research. New York, v. 116, n. 7, p. 2031-2033, 2017.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 2031-2033
Origin http://dx.doi.org/10.1007/s00436-017-5474-2
Access rights ACESSO RESTRITO
Type Article
Web of Science ID WOS:000403940600031
URI https://repositorio.unifesp.br/handle/11600/53609

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