Protein SUMOylation is Involved in Cell-cycle Progression and Cell Morphology in Giardia lamblia

Protein SUMOylation is Involved in Cell-cycle Progression and Cell Morphology in Giardia lamblia

Author Di Genova, Bruno M. Autor UNIFESP Google Scholar
da Silva, Richard C. Autor UNIFESP Google Scholar
da Cunha, Julia P. C. Google Scholar
Gargantini, Pablo R. Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Tonelli, Renata R. Autor UNIFESP Google Scholar
Abstract The unicellular protozoa Giardia lamblia is a food- and waterborne parasite that causes giardiasis. This illness is manifested as acute and self-limited diarrhea and can evolve to long-term complications. Successful establishment of infection by Giardia trophozoites requires adhesion to host cells and colonization of the small intestine, where parasites multiply by mitotic division. The tight binding of trophozoites to host cells occurs by means of the ventral adhesive disc, a spiral array of microtubules and associated proteins such as giardins. In this work we show that knock down of the Small Ubiquitin-like MOdifier (SUMO) results in less adhesive trophzoites, decreased cell proliferation and deep morphological alterations, including at the ventral disc. Consistent with the reduced proliferation, SUMO knocked-down trophozoites were arrested in G1 and in S phases of the cell cycle. Mass spectrometry analysis of anti-SUMO immunoprecipitates was performed to identify SUMO substrates possibly involved in these events. Among the identified SUMOylation targets, -tubulin was further validated by Western blot and confirmed to be a SUMO target in Giardia trophozoites.
Keywords Cell division
cytoskeleton
mass spectrometry
post-translational modification
protozoan
small-ubiquitin-like modifier
tubulin
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
Grant number FAPESP: 2010/15042-2
FAPESP: 2013/04272-5
Date 2017
Published in Journal Of Eukaryotic Microbiology. Hoboken, v. 64, n. 4, p. 491-503, 2017.
ISSN 1066-5234 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent 491-503
Origin http://dx.doi.org/10.1111/jeu.12386
Access rights ACESSO RESTRITO
Type Article
Web of Science ID WOS:000406102000008
URI https://repositorio.unifesp.br/handle/11600/53571

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