The effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentration

The effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentration

Autor Biz, Carolina Autor UNIFESP Google Scholar
Oliveira, Cristiane de Autor UNIFESP Google Scholar
Mattos, Ana Barbosa Marcondes de Autor UNIFESP Google Scholar
Oliveira, Juliana de. Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Metabolic syndrome is associated with an increased risk of developing cardiovascular diseases and Plasminogen activator inhibitor 1 (PAI-1) overexpression may play a significant role in this process. A positive correlation between adipose tissue gene expression of PAI-1 and its serum concentration has been reported. Furthermore, high serum levels of thyroid hormones (T3 and T4) and PAI-1 have been observed in obese children. The present study evaluates the impact of thyroid hormone treatment on white adipose tissue PAI-1 gene expression and its serum concentration. Male Wistar rats (60 days old) were treated for three weeks with T4 (50 µg/day, Hyper) or with saline (control). Additionally, 3T3-L1 adipocytes were treated for 24 h with T4 (100 nM) or T3 (100 nM). PAI-1 gene expression was determined by real-time PCR, while the serum concentration of PAI-1 was measured by ELISA using a commercial kit (Innovative Research, USA). Both the serum concentration of PAI-1 and mRNA levels were similar between groups in retroperitoneal and epididymal white adipose tissue. Using 3T3-L1 adipocytes, in vitro treatment with T4 and T3 increased the gene expression of PAI-1, suggesting non-genomic and genomic effects, respectively. These results demonstrate that thyroid hormones have different effects in vitro and in vivo on PAI-1 gene expression in adipocytes.
Palavra-chave Thyroid hormones
Plasminogen activator inhibitor 1
White adipose tissue
3T3-L1
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: 2006/58628-1
Data de publicação 2009-12-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 12, p. 1163-1166, 2009.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1163-1166
Fonte http://dx.doi.org/10.1590/S0100-879X2009007500013
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000272487500007
SciELO S0100-879X2009001200007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/5344

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