Cafestol, a diterpene molecule found in coffee, induces leukemia cell death

Cafestol, a diterpene molecule found in coffee, induces leukemia cell death

Author Limaa, Caue S. Autor UNIFESP Google Scholar
Spindola, Daniel G. Autor UNIFESP Google Scholar
Bechara, Alexandre Autor UNIFESP Google Scholar
Garcia, Daniel M. Autor UNIFESP Google Scholar
Palmeira-dos-Santos, Caroline Autor UNIFESP Google Scholar
Peixoto-da-Silva, Janaina Autor UNIFESP Google Scholar
Erustes, Adolfo G. Autor UNIFESP Google Scholar
Michelin, Luis F. G. Autor UNIFESP Google Scholar
Pereira, Gustavo J. S. Autor UNIFESP Google Scholar
Smaili, Soraya S. Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Autor UNIFESP Google Scholar
Calgarotto, Andrana K. Autor UNIFESP Google Scholar
Oliveira, Carlos R. Autor UNIFESP Google Scholar
Bincoletto, Claudia Autor UNIFESP Google Scholar
Abstract To evaluate the antitumor properties of Cafestol four leukemia cell lines were used (NB4, K562, HL60 and KG1). Cafestol exhibited the highest cytotoxicity against HL60 and KG1 cells, as evidenced by the accumulation of cells in the sub-G1 fraction, mitochondrial membrane potential reduction, accumulation of cleaved caspase-3 and phosphatidylserine externalization. An increase in CD11b and CD15 differentiation markers with attenuated ROS generation was also observed in Cafestol-treated HL60 cells. These results were similar to those obtained following exposure of the same cell line to cytarabine (Ara-C), an antileukemic drug. Cafestol and Ara-C reduced the clonogenic potential of HL60 cells by 100%, but Cafestol spared murine colony forming unit-granulocyte/macrophage (CFU-GM), which retained their clonogenicity. The co-treatment of Cafestol and Ara-C reduced HL60 cell viability compared with both drugs administered alone. In conclusion, despite the distinct molecular mechanisms involved in the activity of Cafestol and Ara-C, a similar cytotoxicity towards leukemia cells was observed, which suggests a need for prophylactic-therapeutic pre-clinical studies regarding the anticancer properties of Cafestol. (C) 2017 Elsevier Masson SAS. All rights reserved.
Keywords Cafestol
Leukemia
Apoptosis
Cytotoxicity
Clonogenicity
Language English
Sponsor Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) fellowship
Date 2017
Published in Biomedicine & Pharmacotherapy. Paris, v. 92, p. 1045-1054, 2017.
ISSN 0753-3322 (Sherpa/Romeo, impact factor)
Publisher Elsevier France-Editions Scientifiques Medicales Elsevier
Extent 1045-1054
Origin http://dx.doi.org/10.1016/j.biopha.2017.05.109
Access rights Closed access
Type Article
Web of Science ID WOS:000407915600117
URI http://repositorio.unifesp.br/handle/11600/51447

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