Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

Author Marcondes, Rodrigo Rodrigues Google Scholar
Carvalho, Katia Candido Google Scholar
Giannocco, Gisele Autor UNIFESP Google Scholar
Duarte, Daniele Coelho Google Scholar
Garcia, Natalia Google Scholar
Soares-Junior, Jose Maria Google Scholar
Cotrim Guerreiro da Silva, Ismael Dale Autor UNIFESP Google Scholar
Maliqueo, Manuel Google Scholar
Baracat, Edmund Chada Google Scholar
Rosa Maciel, Gustavo Arantes Google Scholar
Abstract OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats.
Keywords Polycystic Ovary Syndrome
Hypothalamus
Animal Models
Kisspeptin
Testosterone
Language English
Sponsor Fundação de Amparo Pesquisa do Estado de São Paulo (FAPESP) -Brazil
Master's degree scholarship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) -Brazil
Grant number FAPESP: 2010/17417-3
FAPESP: 2013/12830-8
CNPq: 134694/2010-4
Date 2017
Published in Clinics. Sao Paulo, v. 72, n. 8, p. 510-514, 2017.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Hospital Clinicas, Univ Sao Paulo
Extent 510-514
Origin http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000800510&lng=en&nrm=iso&tlng=en
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000411803900009
SciELO ID S1807-59322017000800510 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/51409

Show full item record




File

Name: WOS000411803900009.pdf
Size: 529.9Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account