Osteoinductive effects of preoperative dexamethasone in human dental pulp stem cells primary culture

Osteoinductive effects of preoperative dexamethasone in human dental pulp stem cells primary culture

Author Moretti, Rani da Cunha Autor UNIFESP Google Scholar
Duailibi, Monica Talarico Autor UNIFESP Google Scholar
Martins, Paulo Oliveira Google Scholar
dos Santos, Jennifer Adriane Autor UNIFESP Google Scholar
Duailibi, Silvio Eduardo Autor UNIFESP Google Scholar
Abstract Aim: The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques. Our objective was to evaluate the osteoinductive effect of DEX administered as a preoperative medication in primary cell culture of human dental pulp stem cell. Methodology: Cells from the third molar pulp were divided into two experimental groups, each with two preoperative medication protocols used in dental practice and differentiated by the intake of DEX in one of them. The assessment of proliferation, differentiation and viability through trypan blue, methylthiazol tetrazolium, and von Kossa and alizarin red assays, respectively, were held within fixed intervals: 7, 14, 21 and 28 days. Conclusion: This study has shown that DEX may influence in vitro human dental pulp stem cell behavior. Lay abstract: Dexamethasone (DEX) is often used as a preoperative drug in dental surgeries because it reduces pain and has favorable effects on other symptoms caused by the surgery. Additionally, when used in cell culture, its osteoinductor effect is observed. In vitro cell characterization is critical to ensure the development of therapeutic strategies used in tissue engineering. In this sense, this study used two preoperative medication protocols regularly used in dental practice. In Protocol A, patients did not intake DEX

in Protocol B, patients took in tablets of DEX. It was possible to assess in vitro behavior of human dental pulp stem cells by applying those protocols.
Keywords dexamethasone
hDPSC
human dental pulp stem cells
third molar
tissue engineering
Language English
Date 2017
Published in Future Science Oa. London, v. 3, n. 3, p. -, 2017.
ISSN 2056-5623 (Sherpa/Romeo, impact factor)
Publisher Future Sci Ltd
Extent -
Origin http://dx.doi.org/10.4155/fsoa-2016-0083
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000416994800013
URI http://repositorio.unifesp.br/handle/11600/51405

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