Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18

Autor Silva, Marcelo Barbosa da Google Scholar
Thomaz, Luciana Google Scholar
Marques, Alexandre Ferreira Google Scholar
Svidzinski, Artur Estivalet Google Scholar
Nosanchuk, Joshua Daniel Google Scholar
Casadevall, Arturo Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Taborda, Carlos Pelleschi Autor UNIFESP Google Scholar
Instituição Universidade de São Paulo (USP)
Albert Einstein College of Medicine Departments of Medicine and Microbiology and Immunology
Universidade Federal de São Paulo (UNIFESP)
Resumo Paracoccidioides brasiliensis, a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fungal cells to chemically generated nitric oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our results demonstrate that melanized yeast cells were more resistant than nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were added together in this system. Intratracheal infection of BALB/c mice, with melanized yeast cells, resulted in higher lung colony forming units, when compared to nonmelanized yeast cells. Therefore, melanin is a virulence factor of P. brasiliensis.
Assunto melanin
P. brasiliensis
antimicrobial oxidants
anti-CD18 antibody
Idioma Inglês
Data 2009-07-01
Publicado em Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 4, p. 644-648, 2009.
ISSN 0074-0276 (Sherpa/Romeo, fator de impacto)
Editor Instituto Oswaldo Cruz, Ministério da Saúde
Extensão 644-648
Fonte http://dx.doi.org/10.1590/S0074-02762009000400019
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000269466900019
SciELO S0074-02762009000400019 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/handle/11600/5131

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