Characterization of a novel cystatin type 2 from Rhipicephalus microplus midgut

Characterization of a novel cystatin type 2 from Rhipicephalus microplus midgut

Author Cardoso, Thyago H. S. Autor UNIFESP Google Scholar
Lu, Stephen Autor UNIFESP Google Scholar
Gonzalez, Boris R. G. Autor UNIFESP Google Scholar
Torquato, Ricardo J. S. Autor UNIFESP Google Scholar
Tanaka, Aparecida S. Autor UNIFESP Google Scholar
Abstract The Rhipicephalus (Boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. Cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. Recently, our group performed a midgut transcriptome of engorged R. microplus tick, and out of 800-ESTs sequences one cystatin-coding sequence was identified and named Rmcystatin-4. In order to understand the physiological role of Rmcystatin-4, the aim of this work was the expression, purification and functional characterization of a novel type 2 cystatin from the tick R. microplus. Rmcystatin-4 gene expression was identified mostly in tick midgut suggesting its possible role in blood digestion control. Our data showed that rRmcystatin-4 was successfully expressed in active form using Pichia pastoris system and the purified inhibitor presented high selectivity to BmCl-1 (Ki = 0.046 nM). Moreover, rRmcystatin-4 was able to impaired BmCl-1 activity towards bovine hemoglobin. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Keywords Cystatin
Cysteine proteases
Tick
Midgut
Language English
Sponsor Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
INCT-Entomologia Molecular (CNPq)
Grant number FAPESP: 2012/03657-8
FAPESP: 2011/09340-3
CNPq: 308780/2013-2
CNPq: 313073/2013-9
INCT: 465678/2014-9
Date 2017
Published in Biochimie. Paris, v. 140, p. 117-121, 2017.
ISSN 0300-9084 (Sherpa/Romeo, impact factor)
Publisher Elsevier France-Editions Scientifiques Medicales Elsevier
Extent 117-121
Origin http://dx.doi.org/10.1016/j.biochi.2017.07.005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000410870000012
URI http://repositorio.unifesp.br/handle/11600/51307

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