Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Author Chomiski, Veronica Autor UNIFESP Google Scholar
Gragnani, Alfredo Autor UNIFESP Google Scholar
Bonucci, Jessica Autor UNIFESP Google Scholar
Alves Correa, Silvana Aparecida Autor UNIFESP Google Scholar
Ribeiro de Noronha, Samuel Marcos Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Abstract PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene

comparisons 2 and 3 resulted in the same five down-regulated genes

comparison 4 had no significant difference in relative gene expression

comparison 5 showed 26 down-regulated and 7 up-regulated genes

and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
Keywords Burns
Gene Expression
Wound Healing
Keratinocytes
Language English
Sponsor FAPESP [2013/10905-0]
Grant number FAPESP:2013/10905-0
Date 2016
Published in Acta Cirurgica Brasileira. Sao Paulo, v. 31, n. 8, p. 505-512, 2016.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 505-512
Origin http://dx.doi.org/10.1590/S0102-865020160080000002
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000383883900002
SciELO ID S0102-86502016000800505 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/51213

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