Accessing the biocompatibility of layered double hydroxide by intramuscular implantation: histological and microcirculation evaluation

Accessing the biocompatibility of layered double hydroxide by intramuscular implantation: histological and microcirculation evaluation

Author Rodrigues Cunha, Vanessa Roberta Google Scholar
de Souza, Rodrigo Barbosa Autor UNIFESP Google Scholar
Pinto da Fonseca Martins, Ana Maria Cristina Rebello Google Scholar
Jun Koh, Ivan Hong Autor UNIFESP Google Scholar
Leopoldo Constantino, Vera Regina Google Scholar
Abstract Biocompatibility of layered double hydroxides (LDHs), also known as hydrotalcite-like materials or double metal hydroxides, was investigated by in vivo assays via intramuscular tablets implantation in rat abdominal wall. The tablets were composed by chloride ions intercalated into LDH of magnesium/aluminum (Mg2Al-Cl) and zinc/aluminum (Zn2Al-Cl). The antigenicity and tissue integration capacity of LDHs were assessed histologically after 7 and 28 days post-implantation. No fibrous capsule nearby the LDH was noticed for both materials as well any sign of inflammatory reactions. Sidestream Dark Field imaging, used to monitor in real time the microcirculation in tissues, revealed overall integrity of the microcirculatory network neighboring the tablets, with no blood flow obstruction, bleeding and/or increasing of leukocyte endothelial adhesion. After 28 days Mg2Al-Cl promoted multiple collagen invaginations (mostly collagen type-I) among its fragments while Zn2Al-Cl induced predominantly collagen type-III. This work supports previous results in the literature about LDHs compatibility with living matter, endorsing them as functional materials for biomedical applications.
Language English
Sponsor Brazilian agency Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP [2011/50318-1, 2012/12209-9]
Brazilian agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [312384/2013-0]
Grant number FAPESP:2011/50318-1
2012/12209-9
CNPq:312384/2013-0
Date 2016
Published in Scientific Reports. London, v. 6, p. -, 2016.
ISSN 2045-2322 (Sherpa/Romeo, impact factor)
Publisher Nature Publishing Group
Extent -
Origin http://dx.doi.org/10.1038/srep30547
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000380632700001
URI http://repositorio.unifesp.br/handle/11600/51178

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