Analysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosis

Analysis of Gene Expression in the Endocervical Epithelium of Women With Deep Endometriosis

Author Kopelman, Alexander Autor UNIFESP Google Scholar
Girao, Manoel J. B. C. Autor UNIFESP Google Scholar
Bonetti, Tatiana C. S. Autor UNIFESP Google Scholar
Carvalho, Cristina V. Autor UNIFESP Google Scholar
Cotrim Guerreiro da Silva, Ismael Dale Autor UNIFESP Google Scholar
Schor, Eduardo Autor UNIFESP Google Scholar
Abstract Endometriosis affects approximately 12% of reproductive-age women and is currently diagnosed using invasive laparoscopic surgery. Differences in gene expression in the eutopic endometrium between women with and without endometriosis have been reported, and determining the reproducibility of these genetic differences in the endocervical epithelium would represent an important step toward developing novel diagnostic strategies. In this study, we analyzed gene expression in the endocervical epithelium in women with and without moderate or severe endometriosis. Using RT2 Profiler PCR Arrays, we analyzed gene expression in endocervical epithelial cells from women with deep endometriosis (n = 4) and healthy women (n =6). Nine genes were identified as being upregulated: 5 cell cycle genes (cyclin B1 [CCNB1], cyclin G1 [CCNG1], cullin 1 [CUL1], general transcription factor IIH, polypeptide 1 [GTF2H1], and proliferating cell nuclear antigen [PCNA]), 3 cytokine genes (C3, chemokine (C-C motif) ligand 21 [CCL21], and chemokine (C-X-C motif) ligand 14 [CXCL14]) and 1 gene related to dendritic cell pathways (ICAM2), showing that differential gene expression is present in the endocervical epithelium of women with deep endometriosis.
Keywords endometriosis
noninvasive diagnosis
gene expression
endocervix
Language English
Sponsor FAPESP
CNPQ
Date 2016
Published in Reproductive Sciences. Thousand Oaks, v. 23, n. 9, p. 1269-1274, 2016.
ISSN 1933-7191 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Inc
Extent 1269-1274
Origin http://dx.doi.org/10.1177/1933719116638179
Access rights Closed access
Type Article
Web of Science ID WOS:000382544000018
URI http://repositorio.unifesp.br/handle/11600/51137

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