Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

Author Silva Pinhal, Maria Aparecida Autor UNIFESP Google Scholar
Leal Almeida, Maria Carolina Autor UNIFESP Google Scholar
Costa, Alessandra Scorse Autor UNIFESP Google Scholar
Theodoro, Therese Rachell Autor UNIFESP Google Scholar
Serrano, Rodrigo Lorenzetti Autor UNIFESP Google Scholar
Santos Machado Filho, Carlos D'Apparecida Autor UNIFESP Google Scholar
Abstract Background: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Objectives: Evaluate the glycosaminoglycans profile and expression of heparanase in two human cell lines established in culture, immortalized skin keratinocyte (HaCaT) and squamous cell carcinoma (A431) and also investigate the expression of heparanase in basal cell carcinoma, squamous cell carcinoma and eyelid skin of individuals not affected by the disease (control). Methods: Glycosaminoglycans were quantified by electrophoresis and indirect ELISA method. The heparanase expression was analyzed by quantitative RT-PCR (qRTPCR). Results: The A431 strain showed significant increase in the sulfated glycosaminoglycans, increased heparanase expression and decreased hyaluronic acid, comparing to the HaCaT lineage. The mRNA expression of heparanase was significantly higher in Basal cell carcinoma and squamous cell carcinoma compared with control skin samples. It was also observed increased heparanase expression in squamous cell carcinoma compared to the Basal cell carcinoma. Conclusion: The glycosaminoglycans profile, as well as heparanase expression are different between HaCaT and A431 cell lines. The increased expression of heparanase in Basal cell carcinoma and squamous cell carcinoma suggests that this enzyme could be a marker for the diagnosis of such types of non-melanoma cancers, and may be useful as a target molecule for future alternative treatment.
Keywords Carcinoma
basal cell
Carcinoma
squamous cell
Glycosaminoglycans
Hyaluronic acid
Neoplasms
Polymerase chain reaction
Skin neoplasms
Language English
Sponsor FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologia)
CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Date 2016
Published in Anais Brasileiros De Dermatologia. Rio De Janeiro Rj, v. 91, n. 5, p. 595-600, 2016.
ISSN 0365-0596 (Sherpa/Romeo, impact factor)
Publisher Soc Brasileira Dermatologia
Extent 595-600
Origin http://dx.doi.org/10.1590/abd1806-4841.20164957
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000388465100005
SciELO ID S0365-05962016000500595 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/51050

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