Paracoccidioides brasiliensis induces recruitment of alpha 3 and alpha 5 integrins into epithelial cell membrane rafts, leading to cytokine secretion

Paracoccidioides brasiliensis induces recruitment of alpha 3 and alpha 5 integrins into epithelial cell membrane rafts, leading to cytokine secretion

Author Barros, Bianca Carla Silva Campitelli de Autor UNIFESP Google Scholar
Maza, Paloma Korehisa Autor UNIFESP Google Scholar
Alcantara, Cristiane Autor UNIFESP Google Scholar
Suzuki, Erika Autor UNIFESP Google Scholar
Abstract Paracoccidioides brasiliensis is one of the etiological agents of paracoccidioidomycosis, a human systemic mycosis, highly prevalent in Latin America. In the present work, we demonstrate that P. brasiliensis yeasts promote IL-6 and IL-8 secretion by the human lung epithelial cell line A549 in an integrin-dependent manner. In fact, small interfering RNA directed to alpha 3 and alpha 5 integrins decreased IL-6 and IL-8 levels in P. brasiliensis-infected A549 cell cultures. This fungus also led to an increase in the expression of alpha 3 and alpha 5 integrins in this epithelial cell line. In addition, P. brasiliensis yeasts promoted alpha 3 and alpha 5 integrins clustering into A549 cell membrane rafts. Furthermore, epithelial cell membrane raft disruption with nystatin decreased IL-6 and IL-8 levels in P. brasiliensis-A549 cell cultures. Therefore, by increasing host alpha 3 and alpha 5 integrins levels and clustering these receptors into membrane rafts, P. brasiliensis yeasts may modulate host inflammation. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Keywords Membrane Microdomains
Paracoccidioides
Integrins
Interleukin-6
Interleukin-8
Epithelial CellsExtracellular-Matrix Proteins
Lung Adenocarcinoma Cells
Surface Protein
Fibronectin Expression
Mannose Receptor
Fungal Adhesion
Mapk Activation
Lipid Rafts
In-Vitro
Binding
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/22773-6]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Grant number FAPESP: 2011/22773-6
Date 2016
Published in Microbes And Infection. Amsterdam, v. 18, n. 1, p. 68-77, 2016.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier science bv
Extent 68-77
Origin https://doi.org/10.1016/j.micinf.2015.09.003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000368957800008
URI http://repositorio.unifesp.br/handle/11600/49645

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