Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysis

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dc.contributor.author Tim, Carla Roberta
dc.contributor.author Bossini, Paulo Sergio [UNIFESP]
dc.contributor.author Kido, Hueliton Wilian
dc.contributor.author Malavazi, Iran
dc.contributor.author von Zeska Kress, Marcia Regina
dc.contributor.author Carazzolle, Marcelo Falsarella
dc.contributor.author Parizotto, Nivaldo Antonio
dc.contributor.author Renno, Ana Claudia [UNIFESP]
dc.date.accessioned 2019-01-21T10:30:13Z
dc.date.available 2019-01-21T10:30:13Z
dc.date.issued 2016
dc.identifier https://doi.org/10.1016/j.jphotobiol.2015.10.028
dc.identifier.citation Journal Of Photochemistry And Photobiology B-Biology. Lausanne, v. 154, p. 8-15, 2016.
dc.identifier.issn 1011-1344
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49644
dc.description.abstract The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved. en
dc.description.sponsorship FAPESP [2010/15335-0]
dc.description.sponsorship Center for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID) [2013/08293-7]
dc.format.extent 8-15
dc.language.iso eng
dc.publisher Elsevier science sa
dc.relation.ispartof Journal Of Photochemistry And Photobiology B-Biology
dc.rights Acesso restrito
dc.subject Bone Repair en
dc.subject Lllt en
dc.subject Microarray en
dc.subject Gene ExpressionCell-Proliferation en
dc.subject 808 Nm en
dc.subject Repair en
dc.subject Rats en
dc.subject Irradiation en
dc.subject Photobiomodulation en
dc.subject Differentiation en
dc.subject Radiation en
dc.subject Cytokines en
dc.subject Improves en
dc.title Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysis en
dc.type Artigo
dc.description.affiliation Federal University of São Carlos, Department of Physiotherapy, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil
dc.description.affiliation Federal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil
dc.description.affiliation Federal University of São Carlos, Department of Genetics and Evolution, Rod Washington Luis Km 235, São Carlos 13565-905, Brazil
dc.description.affiliation University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical Analysis, Toxicological and Bromatological, Av. do Café 95, Ribeirão Preto, Brazil, 14049-900, Brazil
dc.description.affiliation State University of Campinas, Department of Genetics and Evolution, Cidade Universitária Zeferino Vaz, Campinas 13083-970, Brazil
dc.description.affiliation Brazilian National Center for Research in Energy and Materials, Brazilian Biosciences National Laboratory, Giuseppe Máximo Scolfaro 10.000, Campinas 13083-970, Brazil
dc.description.affiliationUnifesp Federal University of São Paulo, Department of Bioscience, Av. Ana Costa 95, Santos 11050-240, Brazil
dc.description.sponsorshipID FAPESP: 2010/15335-0
dc.description.sponsorshipID FAPESP/CEPID: 2013/08293-7
dc.identifier.doi 10.1016/j.jphotobiol.2015.10.028
dc.description.source Web of Science
dc.identifier.wos WOS:000368954300002



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