Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysis

Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: a microarray analysis

Author Tim, Carla Roberta Google Scholar
Bossini, Paulo Sergio Autor UNIFESP Google Scholar
Kido, Hueliton Wilian Google Scholar
Malavazi, Iran Google Scholar
von Zeska Kress, Marcia Regina Google Scholar
Carazzolle, Marcelo Falsarella Google Scholar
Parizotto, Nivaldo Antonio Google Scholar
Renno, Ana Claudia Autor UNIFESP Google Scholar
Abstract The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved.
Keywords Bone Repair
Lllt
Microarray
Gene ExpressionCell-Proliferation
808 Nm
Repair
Rats
Irradiation
Photobiomodulation
Differentiation
Radiation
Cytokines
Improves
Language English
Sponsor FAPESP [2010/15335-0]
Center for Computational Engineering and Sciences at UNICAMP SP Brazil (FAPESP/CEPID) [2013/08293-7]
Grant number FAPESP: 2010/15335-0
FAPESP/CEPID: 2013/08293-7
Date 2016
Published in Journal Of Photochemistry And Photobiology B-Biology. Lausanne, v. 154, p. 8-15, 2016.
ISSN 1011-1344 (Sherpa/Romeo, impact factor)
Publisher Elsevier science sa
Extent 8-15
Origin https://doi.org/10.1016/j.jphotobiol.2015.10.028
Access rights Closed access
Type Article
Web of Science ID WOS:000368954300002
URI http://repositorio.unifesp.br/handle/11600/49644

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