The diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members

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dc.contributor.author Angel Chiurillo, Miguel
dc.contributor.author Cortez, Danielle R. [UNIFESP]
dc.contributor.author Lima, Fabio M [UNIFESP]
dc.contributor.author Cortez, Caroline [UNIFESP]
dc.contributor.author Luis Ramirez, Jose
dc.contributor.author Martins, Andre G.
dc.contributor.author Serrano, Myrna G.
dc.contributor.author Teixeira, Marta M. G.
dc.contributor.author da Silveira, Jose Franco [UNIFESP]
dc.date.accessioned 2019-01-21T10:30:13Z
dc.date.available 2019-01-21T10:30:13Z
dc.date.issued 2016
dc.identifier https://doi.org/10.1016/j.meegid.2015.11.024
dc.identifier.citation Infection Genetics And Evolution. Amsterdam, v. 37, p. 266-274, 2016.
dc.identifier.issn 1567-1348
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49642
dc.description.abstract Trans-sialidase (TS) is a polymorphic protein superfamily described in members of the protozoan genus Trypanosoma. Of the eight TS groups recently described, TS group I proteins (some of which have catalytic activity) are present in the distantly related Trypanosoma brucei and Trypanosoma cruzi phylogenetic clades, whereas other TS groups have only been described in some species belonging to the T. cruzi clade. In the present study we analyzed the repertoire, distribution and phylogenetic relationships of TS genes among species of the T. cruzi clade based on sequence similarity, multiple sequence alignment and tree-reconstruction approaches using TS sequences obtained with the aid of PCR-based strategies or retrieved from genome databases. We included the following representative isolates of the T. cruzi clade from South America: T. cruzi, T. cruzi Tcbat, Trypanosoma cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli and Trypanosoma conorhini. The cloned sequences encoded conserved TS protein motifs Asp-box and VTVxNVxLYNR but lacked the FRIP motif (conserved in TS group I). The T. conorhini sequences were the most divergent The hybridization patterns of IS probes with chromosomal bands confirmed the abundance of these sequences in species in the T. cruzi clade. Divergence and relationship analysis placed most of the TS sequences in the groups defined in T. cruzi. Further examination of members of TS group II, which includes T. cruzi surface glycoproteins implicated in host cell attachment and invasion, showed that sequences of T. cruzi Tcbat grouped with those of T. cruzi genotype TcI. Our analysis indicates that different members of the T. cruzi clade, with different vertebrate hosts, vectors and pathogenicity, share the extensive expansion and sequence diversification of the TS gene family. Altogether, our results are congruent with the evolutionary history of the T. cruzi clade and represent a contribution to the understanding of the molecular evolution and role of TS proteins in trypanosomes. (C) 2015 Elsevier B.V. All rights reserved. en
dc.description.sponsorship CDCHT-UCLA [007-ME-2007]
dc.description.sponsorship FAPESP [11/51693-0, 11/51475-3]
dc.description.sponsorship Kinetoplastid Genome Sequencing and Analysis Consortium NIH/NHGRI/NIAID [59941]
dc.description.sponsorship National Science Foundation, USA (PI Gregory Buck) [DEB-0830056]
dc.format.extent 266-274
dc.language.iso eng
dc.publisher Elsevier science bv
dc.relation.ispartof Infection Genetics And Evolution
dc.rights Acesso restrito
dc.subject Trypanosoma Cruzi Clade en
dc.subject Trans-Sialidase en
dc.subject Gene Superfamily en
dc.subject Conserved Motif en
dc.subject Phylogeny en
dc.subject DtusCell Invasion en
dc.subject Ribosomal-Rna en
dc.subject In-Vivo en
dc.subject Rangeli en
dc.subject Parasite en
dc.subject Bats en
dc.subject Superfamily en
dc.subject Infection en
dc.subject Lineages en
dc.subject Genome en
dc.title The diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members en
dc.type Artigo
dc.description.affiliation Laboratorio de Genética Molecular “Dr. Yunis-Turbay”, Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado, Barquisimeto 3001, Estado Lara, Venezuela
dc.description.affiliation Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil
dc.description.affiliation Centro de Biotecnología, Fundación Instituto de Estudios Avanzados, Caracas, Venezuela
dc.description.affiliation Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil
dc.description.affiliation Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA
dc.description.affiliationUnifesp Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP 04023-062, Brazil
dc.description.sponsorshipID CDCHT-UCLA 007-ME-2007
dc.description.sponsorshipID FAPESP: 11/51693-0
dc.description.sponsorshipID FAPESP: 11/51475-3
dc.description.sponsorshipID NIH/NHGRI/NIAID: project ID 59941
dc.description.sponsorshipID National Science Foundation, USA: DEB-0830056
dc.identifier.doi 10.1016/j.meegid.2015.11.024
dc.description.source Web of Science
dc.identifier.wos WOS:000368973600035



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