The diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members

The diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members

Author Angel Chiurillo, Miguel Google Scholar
Cortez, Danielle R. Autor UNIFESP Google Scholar
Lima, Fabio M Autor UNIFESP Google Scholar
Cortez, Caroline Autor UNIFESP Google Scholar
Luis Ramirez, Jose Google Scholar
Martins, Andre G. Google Scholar
Serrano, Myrna G. Google Scholar
Teixeira, Marta M. G. Google Scholar
da Silveira, Jose Franco Autor UNIFESP Google Scholar
Abstract Trans-sialidase (TS) is a polymorphic protein superfamily described in members of the protozoan genus Trypanosoma. Of the eight TS groups recently described, TS group I proteins (some of which have catalytic activity) are present in the distantly related Trypanosoma brucei and Trypanosoma cruzi phylogenetic clades, whereas other TS groups have only been described in some species belonging to the T. cruzi clade. In the present study we analyzed the repertoire, distribution and phylogenetic relationships of TS genes among species of the T. cruzi clade based on sequence similarity, multiple sequence alignment and tree-reconstruction approaches using TS sequences obtained with the aid of PCR-based strategies or retrieved from genome databases. We included the following representative isolates of the T. cruzi clade from South America: T. cruzi, T. cruzi Tcbat, Trypanosoma cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli and Trypanosoma conorhini. The cloned sequences encoded conserved TS protein motifs Asp-box and VTVxNVxLYNR but lacked the FRIP motif (conserved in TS group I). The T. conorhini sequences were the most divergent The hybridization patterns of IS probes with chromosomal bands confirmed the abundance of these sequences in species in the T. cruzi clade. Divergence and relationship analysis placed most of the TS sequences in the groups defined in T. cruzi. Further examination of members of TS group II, which includes T. cruzi surface glycoproteins implicated in host cell attachment and invasion, showed that sequences of T. cruzi Tcbat grouped with those of T. cruzi genotype TcI. Our analysis indicates that different members of the T. cruzi clade, with different vertebrate hosts, vectors and pathogenicity, share the extensive expansion and sequence diversification of the TS gene family. Altogether, our results are congruent with the evolutionary history of the T. cruzi clade and represent a contribution to the understanding of the molecular evolution and role of TS proteins in trypanosomes. (C) 2015 Elsevier B.V. All rights reserved.
Keywords Trypanosoma Cruzi Clade
Gene Superfamily
Conserved Motif
DtusCell Invasion
Language English
Sponsor CDCHT-UCLA [007-ME-2007]
FAPESP [11/51693-0, 11/51475-3]
Kinetoplastid Genome Sequencing and Analysis Consortium NIH/NHGRI/NIAID [59941]
National Science Foundation, USA (PI Gregory Buck) [DEB-0830056]
Grant number CDCHT-UCLA 007-ME-2007
FAPESP: 11/51693-0
FAPESP: 11/51475-3
NIH/NHGRI/NIAID: project ID 59941
National Science Foundation, USA: DEB-0830056
Date 2016
Published in Infection Genetics And Evolution. Amsterdam, v. 37, p. 266-274, 2016.
ISSN 1567-1348 (Sherpa/Romeo, impact factor)
Publisher Elsevier science bv
Extent 266-274
Access rights Closed access
Type Article
Web of Science ID WOS:000368973600035

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