Expression of p53, her2 and ki67 proteins in rats subjected to bladder augmentation with stomach, colon and ileum

Expression of p53, her2 and ki67 proteins in rats subjected to bladder augmentation with stomach, colon and ileum

Author de Miranda, Edinaldo Goncalves Google Scholar
Branco Couto de Miranda, Celina Teresa Castelo Google Scholar
Paraiba Lopes, Antonio Igor Google Scholar
Santana, Diogenes Miranda Google Scholar
Pinheiro Lemos Filho, Joao Emilio Google Scholar
Diniz, Thiago Pereira Google Scholar
Silva, Jose Figueredo Google Scholar
Waisberg, Jaques Autor UNIFESP Google Scholar
Abstract PURPOSE: To study the expression of HER2, p53 and Ki67 proteins in cystoplasties. METHODS: Sixty rats were distributed randomly into three groups of 20 animals. Bladder augmentation was held to increase with ileum (Group I), colon (Group II) and stomach (Group III). Tissue samples of neobladder was collected from each rat to its own control. The animals were sacrificed after 12 weeks. The neobladder was withdrawn for immunohistochemitry analysis of p53, HER2 and Ki67 expression. Wilcoxon and Mann-Whitney tests were used for statistical study. RESULTS: There were no significant changes in the expression of p53 and HER2 proteins. It was observed significant increase (p<0.0001) in Ki67 expression in all groups, when compared with their respective controls. When the study groups were compared with each other, there was increase of cell proliferation in the largest gastrocystoplasties in respect of ileocystoplasties (p=0.004) and colocystoplasties (p=0.003). CONCLUSION: We observed significant increase of cell proliferation characterized by Ki67 protein in the digestive tract of the ileocystoplasties, the colocystoplasties and the gastrocystoplasties and this increase was significantly greater in gastrocystoplasties.
Keywords Urinary Bladder
Neoplasms
Genes
P53
RatsAutoaugmentation
Cystoplasty
Growth
Tissue
Tumor
Language English
Sponsor Universidade Estadual do Piaui (FACIME/UESPI)
Date 2016
Published in Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 44-52, 2016.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta cirurgica brasileira
Extent 44-52
Origin http://dx.doi.org/10.1590/S0102-865020160010000007
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000369657900007
SciELO ID S0102-86502016000100044 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/49621

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