Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats

Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats

Author Fiorindo Faria, Danilo Jose Autor UNIFESP Google Scholar
Simoes, Manuel de Jesus Autor UNIFESP Google Scholar
Teixeira, Luciana Cristina Autor UNIFESP Google Scholar
Pinheiro Faria, Angela Tucci Google Scholar
Simoes Ulhoa Cintra, Alvaro Edmundo Autor UNIFESP Google Scholar
Martins, Jose Luiz Autor UNIFESP Google Scholar
Abstract PURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADR

Group B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p <= 0.05*. RESULTS: 81 fetuses were harvested. The number of fetuses

number of ARMs

mean (Delta%) (+/- SD) were determined to be, respectively: ADR -41[29

65%(+/- 37%)] versus FA+ADR - 40[04

16%(+/- 36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (mu m) of ASSE (+/- SD) and IE (+/- SD) were measured, respectively: ADR -[25.98(+/- 0.74) and 19.48(+/- 1.68)] versus FA+ADR -[24.74(+/- 0.91) and 24.80(+/- 0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced.
Keywords Anal Canal
Anus
Imperforate
Doxorubicin
Folic Acid
RatsNeural-Tube Defects
Embryonic-Development
Hindgut Development
Mouse Model
Supplementation
Notochord
Ethylenethiourea
Expression
Pregnancy
Anomalies
Language English
Date 2016
Published in Acta Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta cirurgica brasileira
Extent 22-27
Origin http://dx.doi.org/10.1590/S0102-865020160010000004
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000369657900004
SciELO ID S0102-86502016000100022 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/49619

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