Studies of molecular changes in intervertebral disc degeneration in animal model

Studies of molecular changes in intervertebral disc degeneration in animal model

Author de Campos, Marcelo Ferraz Google Scholar
de Oliveira, Cintia Pereira Google Scholar
Neff, Charles Benjamin Google Scholar
de Toledo Correa, Olga Maria Google Scholar
Silva Pinhal, Maria Aparecida Autor UNIFESP Google Scholar
Reis Rodrigues, Luciano Miller Google Scholar
Abstract Objective: To evaluate the structural and molecular changes in the extracellular matrix (ECM) during the process of intervertebral disc degeneration, using animal model. Methods: Wistar rats underwent intervertebral disc degeneration through 20-gauge needle puncture, and 360 degrees rotation applied for 30 sec, representing the degenerated group, while control group was not submitted to this procedure. Histological parameters and expression of extracellular matrix molecules were evaluated in the 15th and 28th days after degenerative induction. Results: Fifteen days after the induction of intervertebral disc degeneration, significant changes were observed, such as reduction in the expression metalloprotease-9 (MMP9) and interleukins (IL-6 and IL-10). There was a significant increase in the expression of vascular endothelial growth factor (VEGF) and caspase-3. However, different alterations in the ECM were observed at 28 days, the level of collagen I, metallo-protease-2 (MMP2) and caspase-3 were enhanced. Furthermore, expression of heparanase isoforms (HPSE1 and HPSE2) mRNA were increased in the degenerative intervertebral disc. Conclusion: The different profiles of ECM molecules observed during the intervertebral disc degeneration suggest that molecular processes such as ECM remodeling, neovascularization, apoptosis and inflammation occur. Experimental Study.
Keywords Intervertebral Disc Degeneration
Neovascularization, Pathologic
ApoptosisAnnulus Fibrosus Cells
Needle Puncture
Rabbit Model
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Date 2016
Published in Acta Ortopedica Brasileira. Sao paulo sp, v. 24, n. 1, p. 16-21, 2016.
ISSN 1413-7852 (Sherpa/Romeo, impact factor)
Publisher Atha comunicacao & editora
Extent 16-21
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000372326100002
SciELO ID S1413-78522016000100016 (statistics in SciELO)

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