Biointeractions of ultrasmall glutathione-coated gold nanoparticles: effect of small size variations

Biointeractions of ultrasmall glutathione-coated gold nanoparticles: effect of small size variations

Author Sousa, Alioscka A. Autor UNIFESP Google Scholar
Hassan, Sergio A. Google Scholar
Knittel, Luiza L. Autor UNIFESP Google Scholar
Balbo, Andrea Google Scholar
Aronova, Maria A. Google Scholar
Brown, Patrick H. Google Scholar
Schuckc, Peter Google Scholar
Leapman, Richard D. Google Scholar
Abstract Recent in vivo studies have established ultrasmall (<3 nm) gold nanoparticles coated with glutathione (AuGSH) as a promising platform for applications in nanomedicine. However, systematic in vitro investigations to gain a more fundamental understanding of the particles' biointeractions are still lacking. Herein we examined the behavior of ultrasmall AuGSH in vitro, focusing on their ability to resist aggregation and adsorption from serum proteins. Despite having net negative charge, AuGSH particles were colloidally stable in biological media and able to resist binding from serum proteins, in agreement with the favorable bioresponses reported for AuGSH in vivo. However, our results revealed disparate behaviors depending on nanoparticle size: particles between 2 and 3 nm in core diameter were found to readily aggregate in biological media, whereas those strictly under 2 nm were exceptionally stable. Molecular dynamics simulations provided microscopic insight into interparticle interactions leading to aggregation and their sensitivity to the solution composition and particle size. These results have important implications, in that seemingly small variations in size can impact the biointeractions of ultrasmall AuGSH, and potentially of other ultrasmall nanoparticles as well.
Keywords Side-Chain Interactions
Analytical Ultracentrifugation
Renal Clearance
In-Vivo
Dependent Manner
Biodistribution
Nanoclusters
Toxicity
Plasma
Corona
Language English
Sponsor Sao Paulo Research Foundation, Sao Paulo, Brazil [FAPESP: 2013/18481-5]
Brazilian National Council for Scientific and Technological Development [CNPq: 476784/2013-1]
National Institute of Biomedical Imaging and Bioengineering
Center for Information Technology, National Institutes of Health, Bethesda, U.S.A.
Grant number FAPESP: 2013/18481-5
CNPq: 476784/2013-1
Date 2016
Published in Nanoscale. Cambridge, v. 8, n. 12, p. 6577-6588, 2016.
ISSN 2040-3364 (Sherpa/Romeo, impact factor)
Publisher Royal soc chemistry
Extent 6577-6588
Origin http://dx.doi.org/10.1039/c5nr07642k
Access rights Closed access
Type Article
Web of Science ID WOS:000372851500043
URI http://repositorio.unifesp.br/handle/11600/49562

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