Ionic and biochemical characterization of bovine intervertebral disk

Ionic and biochemical characterization of bovine intervertebral disk

Author Martins, Delio Eulalio Autor UNIFESP Google Scholar
Medeiros, Valquiria Pereira Google Scholar
Demerov, Gabriela Feitosa Autor UNIFESP Google Scholar
Accardo, Camila Melo Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Wajchenberg, Marcelo Autor UNIFESP Google Scholar
Reginato, Rejane Daniele Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Puertas, Eduardo Barros Autor UNIFESP Google Scholar
Faloppa, Flavio Autor UNIFESP Google Scholar
Abstract Introduction: Intervertebral disks have been associated with low back pain, and many therapies have been proposed for its treatment. The cellular and molecular knowledge of intervertebral disks composition and precise methods to quantify disk components are important for any type of proposed therapy. Thus, the aim of this study was to correlate glycosaminoglycans presence with the quantitation of cells, ions and collagen fiber distributions in different intervertebral disk sections. Methods: In total, 14 intervertebral disks were used from cattle. All of the disks were dehydrated, separated in seven sections and digested in sodium-free papain buffer. Glycosaminoglycan measurements were performed in the samples according to agarose electrophoresis method

total cells were measured using the PicoGreen (R) technique, ions were quantified, and collagen fiber birefringence was analyzed with polarized light. Results: Cations Na+ and K+ are more concentrate in the nucleus (Na+ = 1688.50 +/- 110 mmol/L

K+ = 111.9 +/- 28 mmol/L) of intervertebral disks than the annulus (Na+ = 652.80 +/- 75 mmol/L

K+ = 55.6 +/- 8 mmol/L). A negative correlation between cells number and sodium/potassium was observed (p < 0.001) Additionally, thin collagen fibers were largest in the nucleus, similar to hyaluronate distribution. Conclusions: The results suggest that annulus fibrosus cells are also sensitive to changes in ionic concentrations such as nucleus pulposus cells. Additionally, hyaluronate is related to thin collagen fibers type II.
Keywords Collagen
Glycosaminoglycans
Hyaluronic Acid
Intervertebral Disk
Potassium
SodiumExtracellular-Matrix
End-Plate
Acidic Mucopolysaccharides
Cartilage
Dna
Hoechst-33258
Hyaluronan
Therapy
Density
Assay
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2009/54793-6]
CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Grant number FAPESP: 2009/54793-6
Date 2016
Published in Connective Tissue Research. Philadelphia, v. 57, n. 3, p. 212-219, 2016.
ISSN 0300-8207 (Sherpa/Romeo, impact factor)
Publisher Taylor & francis inc
Extent 212-219
Origin https://doi.org/10.3109/03008207.2016.1140751
Access rights Closed access
Type Article
Web of Science ID WOS:000375235800005
URI http://repositorio.unifesp.br/handle/11600/49522

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