Previous exercise training increases levels of ppar-alpha in long-term post-myocardial infarction in rats, which is correlated with better inflammatory response

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dc.contributor.author Higuchi Santos, Marilia Harumi
dc.contributor.author Higuchi, Maria de Lourdes
dc.contributor.author Tucci, Paulo J. F. [UNIFESP]
dc.contributor.author Garavelo, Sherrira M.
dc.contributor.author Reis, Marcia M.
dc.contributor.author Antonio, Ednei L. [UNIFESP]
dc.contributor.author Serra, Andrey J.
dc.contributor.author Maranhao, Raul Cavalcante
dc.date.accessioned 2019-01-21T10:29:59Z
dc.date.available 2019-01-21T10:29:59Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.6061/clinics/2016(03)08
dc.identifier.citation Clinics. Sao paulo, v. 71, n. 3, p. 163-168, 2016.
dc.identifier.issn 1807-5932
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49517
dc.description.abstract OBJECTIVE: Exercise is a protective factor for cardiovascular morbidity and mortality, with unclear mechanisms. Changing the myocardial metabolism causes harmful consequences for heart function and exercise contributes to metabolic adjustment modulation. Peroxisome proliferator-activated receptors (PPARs) are also myocardium metabolism regulators capable of decreasing the inflammatory response. We hypothesized that PPAR-alpha is involved in the beneficial effects of previous exercise on myocardial infarction (MI) and cardiac function, changing the expression of metabolic and inflammatory response regulators and reducing myocardial apoptosis, which partially explains the better outcome. METHODS AND RESULTS: Exercised rats engaged in swimming sessions for 60 min/day, 5 days/week, for 8 weeks. Both the exercised rats and sedentary rats were randomized to MI surgery and followed for 1 week (EI1 or SI1) or 4 weeks (EI4 or SI4) of healing or to sham groups. Echocardiography was employed to detect left ventricular function and the infarct size. Additionally, the TUNEL technique was used to assess apoptosis and immunohistochemistry was used to quantitatively analyze the PPAR-alpha, TNF-alpha and NF-kappa B antigens in the infarcted and non-infarcted myocardium. MI-related mortality was higher in SI4 than in EI4 (25% vs 12%), without a difference in MI size. SI4 exhibited a lower shortening fraction than EI4 did (24% vs 35%) and a higher apoptosis/area rate (3.97 +/- 0.61 vs 1.90 +/- 1.82) in infarcted areas (both p=0.001). Immunohistochemistry also revealed higher TNF-alpha levels in SI1 than in EI1 (9.59 vs 4.09, p<0.001) in infarcted areas. In non-infarcted areas, EI4 showed higher levels of TNF-alpha and positive correlations between PPAR-alpha and NF-kappa B (r=0.75, p=0.02), in contrast to SI4 (r=0.05, p=0.87). CONCLUSION: Previously exercised animals had better long-term ventricular function post-MI, in addition to lower levels of local inflammatory markers and less myocardial apoptosis, which seemed to be related to the presence of PPAR-alpha. en
dc.description.sponsorship Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [06/50489-2]
dc.description.sponsorship Zerbini Foundation
dc.format.extent 163-168
dc.language.iso eng
dc.publisher Hospital clinicas, univ sao paulo
dc.relation.ispartof Clinics
dc.rights Acesso aberto
dc.subject Exercise Training en
dc.subject Myocardial Infarction en
dc.subject Ppar-Alpha en
dc.subject Apoptosis en
dc.subject InflammationAcute Myocardial-Infarction en
dc.subject Activated Receptor-Alpha en
dc.subject Left-Ventricular Function en
dc.subject Improves en
dc.subject Hypertrophy en
dc.subject Protection en
dc.subject Disease en
dc.subject Death en
dc.title Previous exercise training increases levels of ppar-alpha in long-term post-myocardial infarction in rats, which is correlated with better inflammatory response en
dc.type Artigo
dc.description.affiliation Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Instituto do Coração (InCor), Laboratório de Patologia Cardíaca, São Paulo/, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP), Cardiologia, São Paulo, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP), Fisiologia Cardíaca, São Paulo, SP, Brazil
dc.description.affiliation Universidade Nove Julho, Programa de Pós Graduação em Biofotônica Aplicada às Ciências da Saúde, São Paulo/, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo (UNIFESP), Cardiologia, São Paulo, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo (UNIFESP), Fisiologia Cardíaca, São Paulo, SP, Brazil
dc.description.sponsorshipID FAPESP: 06/50489-2
dc.identifier.file S1807-59322016000300163.pdf
dc.identifier.scielo S1807-59322016000300163
dc.identifier.doi 10.6061/clinics/2016(03)08
dc.description.source Web of Science
dc.identifier.wos WOS:000374484800008



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