Analysis of the ki-67 index in the vaginal epithelium of castrated rats treated with tamoxifen

Analysis of the ki-67 index in the vaginal epithelium of castrated rats treated with tamoxifen

Author Nery-Aguiar, Afif Rieth Google Scholar
Aguiar, Yousef Qathaf Google Scholar
Conde Junior, Airton Mendes Google Scholar
Alencar, Airlane Pereira Google Scholar
Tavares, Cleciton Braga Google Scholar
Lopes-Costa, Pedro Vitor Google Scholar
Nazario, Afonso Celso Autor UNIFESP Google Scholar
da Silva, Benedito Borges Google Scholar
Abstract OBJECTIVES: Vaginal atrophy and breast cancer are common conditions in postmenopausal women and tamoxifen is the standard endocrine treatment for hormone-sensitive tumors. The present study aimed to assess the effect of tamoxifen on Ki-67 protein expression in the vaginal epithelium of castrated rats. MATERIAL AND METHODS: Forty Wistar-Hannover adult, virgin, castrated rats were randomly divided into two groups, group I (control, n= 20) and group II (tamoxifen, n= 20), receiving 0.5 ml of propylene glycol and 250 mu g of tamoxifen diluted in 0.5 ml of propylene glycol, respectively, daily by gavage for 30 days. On the 31st day, the rats were euthanized and their vaginas were removed and fixed in 10% buffered formalin for the immunohistochemical study of Ki-67 protein expression. Data were analyzed by the Levene and Student's t tests (p<0.05). RESULTS: The mean index of Ki-67 expression in the rat vagina of groups I and II was 4.04 +/- 0.96 and 26.86 +/- 2.19, respectively (p<0.001). CONCLUSIONS: According to the results of the present study, tamoxifen, at the dose and treatment length used, induced a significant increase in the cell proliferation of the vaginal mucosa in castrated rats, as evaluated by Ki-67 protein expression.
Keywords Tamoxifen
Rat
Vagina
Proliferation
Ki-67Breast-Cancer
Endometrium
Prevention
Raloxifene
Expression
Women
Language English
Date 2016
Published in Clinics. Sao paulo, v. 71, n. 2, p. 90-93, 2016.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Hospital clinicas, univ sao paulo
Extent 90-93
Origin http://dx.doi.org/10.6061/clinics/2016(02)07
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000374485200007
SciELO ID S1807-59322016000200090 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/49516

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