The plant-derived bauhinia bauhinioides kallikrein proteinase inhibitor (rbbki) attenuates elastase-induced emphysema in mice

The plant-derived bauhinia bauhinioides kallikrein proteinase inhibitor (rbbki) attenuates elastase-induced emphysema in mice

Author Martins-Olivera, Bruno Tadeu Google Scholar
Almeida-Reis, Rafael Google Scholar
Theodoro-Junior, Osmar Aparecido Google Scholar
Oliva, Leandro Vilela Google Scholar
dos Santos Nunes, Natalia Neto Autor UNIFESP Google Scholar
Olivo, Clarice Rosa Google Scholar
de Brito, Marlon Vilela Autor UNIFESP Google Scholar
Prado, Carla Maximo Autor UNIFESP Google Scholar
Leick, Edna Aparecida Google Scholar
Martins, Milton de Arruda Google Scholar
Vilela Oliva, Maria Luiza Autor UNIFESP Google Scholar
Righetti, Renato Fraga Google Scholar
Lopes Calvo Tiberio, Iolanda de Fatima Google Scholar
Abstract Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2 alpha, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.
Keywords Induced Pulmonary-Emphysema
Rho-Kinase Inhibition
Chronic Inflammation
Plasma Kallikrein
Animal-Models
Copd
Lung
Stress
Tissue
Proliferation
Language English
Sponsor CNPq
FAPESP [2013/17944-1]
LIM-20 HC/FMUSP
Grant number FAPESP: 2013/17944-1
Date 2016
Published in Mediators Of Inflammation. New york, 2016.
ISSN 0962-9351 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Origin http://dx.doi.org/10.1155/2016/5346574
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000381139500001
URI http://repositorio.unifesp.br/handle/11600/49448

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