Comparative cytogenetic analysis in 13 tortoise beetles (coleoptera: chrysomelidae: cassidinae) from brazil

Comparative cytogenetic analysis in 13 tortoise beetles (coleoptera: chrysomelidae: cassidinae) from brazil

Author Lopes, Amalia T. Google Scholar
Fernandes, Flavia R. Google Scholar
Schneider, Marielle C. Autor UNIFESP Google Scholar
Abstract In the present work, we have characterized the chromosomes of 13 Cassidinae beetles, belonging to four tribes, the broad aim being to increase the cytogenetic data and establish the mechanisms involved in chromosome evolution of this subfamily, which appear to be conserved karyotypically, i.e. 2n = 16 + Xy(p). The analysis of mitotic and meiotic cells revealed a high diversity of diploid numbers (2n = 18, 2n = 22, 2n = 26, 2n = 32, 2n = 36, 2n = 40, 2n = 42), and the presence of sex chromosome system of the Xy(p) type in most species, with the exception of two representatives that exhibited Xy(r) and XY systems. C-banding showed constitutive heterochromatin predominantly localized in the pericentromeric region of the chromosomes, but differences regarding the number of chromosomes with positive C-bands, intensity of the blocks, and presence of additional bands in autosomes and/or sex chromosomes were observed among the species investigated. Our data revealed that the karyotype 2n = 16 + Xy(p) does not occur in all 13 tribes of the Cassidinae characterized cytogenetically, seeming to be only a shared feature among the species of the Cassidini. Variations in the C-band pattern, mainly in closely related species, suggest that the interspecific karyotype diversification occurred as a result of changes in the quantity and distribution of constitutive heterochromatin. The occurrence of the Xy(p) sex chromosome system in the tribe Mesomphaliini, which showed the highest diversity of simple and multiple systems among the coleopteran as a whole, reinforces the view that derived systems originated by chromosome rearrangements involving the Xy(p) ancestral system.
Keywords Coleoptera
Chrysomelidae
C-Band
Chromosome
Karyotype
Meiosis
Sex Chromosome System
Constitutive HeterochromatinSex-Determining Mechanism
Chromosome-Number
Repetitive Dna
Elateridae
Hispinae
Heterochromatin
Scarabaeidae
Conoderini
Alticinae
Evolution
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP [2011/21643-1, 2012/12619-2]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq [302416/2013-7]
Grant number FAPESP: 2011/21643-1
FAPESP: 2012/12619-2
CNPq: 302416/2013-7
Date 2016
Published in European Journal Of Entomology. Ceske budejovice, v. 113, p. 352-363, 2016.
ISSN 1802-8829 (Sherpa/Romeo, impact factor)
Publisher Assoc Arquivos Neuro- Psiquiatria
Extent 352-363
Origin http://dx.doi.org/10.14411/eje.2016.046
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000380706700042
URI http://repositorio.unifesp.br/handle/11600/49444

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