Calcium phosphate fibers coated with collagen: in vivo evaluation of the effects on bone repair

Calcium phosphate fibers coated with collagen: in vivo evaluation of the effects on bone repair

Author Ueno, Fabio Roberto Autor UNIFESP Google Scholar
Kido, Hueliton Wilian Autor UNIFESP Google Scholar
Granito, Renata Neves Autor UNIFESP Google Scholar
Gabbai-Armelin, Paulo Roberto Autor UNIFESP Google Scholar
Paiva Magri, Angela Maria Autor UNIFESP Google Scholar
Fernandes, Kelly Rosseti Autor UNIFESP Google Scholar
da Silva, Antonio Carlos Google Scholar
Correa Braga, Francisco Jose Google Scholar
Muniz Renno, Ana Claudia Autor UNIFESP Google Scholar
Abstract The aim of this study was to assess the characteristics of the CaP/Col composites, in powder and fiber form, via scanning electron microscopy (SEM), pH and calcium release evaluation after immersion in SBF and to evaluate the performance of these materials on the bone repair process in a tibial bone defect model. For this, four different formulations (CaP powder -CaPp, CaP powder with collagen -CaPp/Col, CaP fibers - CaPf and CaP fibers with collagen - CaPf/Col) were developed. SEM images indicated that both material forms were successfully coated with collagen and that CaPp and CaPf presented HCA precursor crystals on their surface. Although presenting different forms, FTIR analysis indicated that CaPp and CaPf maintained the characteristic peaks for this class of material. Additionally, the calcium assay study demonstrated a higher Ca uptake for CaPp compared to CaPf for up to 5 days. Furthermore, pH measurements revealed that the collagen coating prevented the acidification of the medium, leading to higher pH values for CaPp/Col and CaPf/Col. The histological analysis showed that CaPf/Col demonstrated a higher amount of newly formed bone in the region of the defect and a reduced presence of material. In summary, the results indicated that the fibrous CaP enriched with the organic part (collagen) glassy scaffold presented good degradability and bone-forming properties and also supported Runx2 and RANKL expression. These results show that the present CaP/Col fibrous composite may be used as a bone graft for inducing bone repair.
Keywords Composite
Calcium Phosphate
Collagen
Fibers
Bone RepairBioactive Glass
Vitro Degradation
Composites
Osteoblast
Scaffold
Cement
Regeneration
Biocompatibility
Hydroxyapatite
Mineralization
Language English
Date 2016
Published in Bio-Medical Materials And Engineering. Amsterdam, v. 27, n. 43161, p. 259-273, 2016.
ISSN 0959-2989 (Sherpa/Romeo, impact factor)
Publisher Cadernos Saude Publica
Extent 259-273
Origin http://dx.doi.org/10.3233/BME-161581
Access rights Closed access
Type Article
Web of Science ID WOS:000383187600012
URI http://repositorio.unifesp.br/handle/11600/49406

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