Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

Author Pedroso Sakoda, Camila Pivari Autor UNIFESP Google Scholar
de Toledo, Alessandra Choqueta Google Scholar
Perini, Adenir Google Scholar
Pinheiro, Nathalia Montouro Google Scholar
Hiyane, Meire Ioshie Google Scholar
Grecco, Simone dos Santos Autor UNIFESP Google Scholar
Lopes Calvo Tiberio, Iolanda de Fatima Google Scholar
Saraiva Camara, Niels Olsen Google Scholar
Martins, Milton de Arruda Google Scholar
Ghilardi Lago, Joao Henrique Autor UNIFESP Google Scholar
Righetti, Renato Fraga Google Scholar
Prado, Carla Maximo Autor UNIFESP Google Scholar
Abstract Background and purpose: Asthma is a disease of high prevalence and morbidity that generates high costs in hospitalization and treatment. Although the airway is involved in the physiopathology of asthma, there is also evidence of the importance of vascular and lung parenchyma inflammation and remodeling, which can contribute to the functional pulmonary alterations observed in asthmatic patients. Our aim was to evaluate treatment using sakuranetin, a flavone isolated from the twigs of Baccharis retusa (Asteraceae), on vascular and lung parenchyma alterations in an experimental murine model of asthma. Methods: Male BALB/c mice were subjected to a sensitization protocol with ovalbumin for 30 days and were treated with or without sakuranetin (20 mg/kg/mice) or dexamethasone (5 mg/kg/mice)

then, the lungs were collected for histopathological analysis. We evaluated extracellular matrix remodeling (collagen and elastic fibers), inflammation (eosinophils and NF-kB) and oxidative stress (8-isoprostane) in the pulmonary vessels and lung parenchyma. The thickness of the vascular wall was quantified, as well as the vascular endothelial growth factor (VEGF) levels. Results: We demonstrated that sakuranetin reduced the number of eosinophils and elastic fibers in both the pulmonary vessels and the lung parenchyma, probably due to a reduction of oxidative stress and of the transcription factor NF-kB and VEGF levels in the lung. In addition, it reduced the thickness of the pulmonary vascular wall. The treatment had no effect on the collagen fibers. In most of the parameters, the effect of sakuranetin was similar to the dexamethasone effect. Conclusions and implications: Sakuranetin had anti-inflammatory and antioxidant effects, preventing vascular and distal parenchyma changes in this experimental model of asthma. (C) 2016 Elsevier GmbH. All rights reserved.
Keywords Bronchial Asthma
Sakuranetin
Remodeling
Oxidative Stress
Peribronchial Vessels
Lung ParenchymaNf-Kappa-B
Airway Inflammation
Oxidative Stress
Mast-Cells
Asthma
Flavonoids
Inhibition
Asteraceae
Luteolin
Disease
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/14831-3, 2008/55359-5, 2014/25689-4]
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [304465/2012-7, 476877/2012-1]
Grant number FAPESP: 2010/14831-3
FAPESP: 2008/55359-5
FAPESP: 2014/25689-4
CNPq: 304465/2012-7
CNPq: 476877/2012-1
Date 2016
Published in Acta Histochemica. Jena, v. 118, n. 6, p. 615-624, 2016.
ISSN 0065-1281 (Sherpa/Romeo, impact factor)
Publisher Cadernos Saude Publica
Extent 615-624
Origin https://doi.org/10.1016/j.acthis.2016.07.001
Access rights Closed access
Type Article
Web of Science ID WOS:000383010800008
URI http://repositorio.unifesp.br/handle/11600/49405

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