Hpv genotyping and p16 expression in xingu indigenous park, brazil

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dc.contributor.author Freitas, V. G. [UNIFESP]
dc.contributor.author Focchi, G. R. [UNIFESP]
dc.contributor.author Pereira, E. R. [UNIFESP]
dc.contributor.author Levi, J. E.
dc.contributor.author Speck, N. M. G. [UNIFESP]
dc.contributor.author Ribalta, J. C. [UNIFESP]
dc.date.accessioned 2019-01-21T10:29:47Z
dc.date.available 2019-01-21T10:29:47Z
dc.date.issued 2016
dc.identifier http://dx.doi.org/10.4238/gmr.15036840
dc.identifier.citation Genetics And Molecular Research. Ribeirao preto, v. 15, n. 3, p. UNSP 15036840, 2016.
dc.identifier.issn 1676-5680
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/49389
dc.description.abstract The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec (R) Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 +/- 15.89 years) and parity ranged from 1-11 (5.92 +/- 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant en
dc.description.abstract it was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotype en
dc.description.abstract however, more studies are necessary to further our understanding of this restricted group. en
dc.description.sponsorship Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) grant
dc.format.extent UNSP 15036840
dc.language.iso eng
dc.publisher Univ Fed Sao Carlos, Dept Engenharia Materials
dc.relation.ispartof Genetics And Molecular Research
dc.rights Acesso aberto
dc.subject P16 Protein en
dc.subject P16 Expression en
dc.subject Human Papillomavirus en
dc.subject Indigenous en
dc.subject Xingu en
dc.subject GenotypeCervical Intraepithelial Neoplasia en
dc.subject Human-Papillomavirus Genotypes en
dc.subject Cancer en
dc.subject Women en
dc.subject Infection en
dc.subject Lesions en
dc.subject Diagnosis en
dc.subject Risk en
dc.subject Prevalence en
dc.subject Prevention en
dc.title Hpv genotyping and p16 expression in xingu indigenous park, brazil en
dc.type Artigo
dc.description.affiliation Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliation Departamento de Patologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliation Departamento de Medicina Preventiva, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliation Instituto de Medicina Tropical da Universidade de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifesp Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifesp Departamento de Patologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUnifesp Departamento de Medicina Preventiva, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
dc.identifier.doi 10.4238/gmr.15036840
dc.description.source Web of Science
dc.identifier.wos WOS:000384881100006



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