Hpv genotyping and p16 expression in xingu indigenous park, brazil

Hpv genotyping and p16 expression in xingu indigenous park, brazil

Author Freitas, V. G. Autor UNIFESP Google Scholar
Focchi, G. R. Autor UNIFESP Google Scholar
Pereira, E. R. Autor UNIFESP Google Scholar
Levi, J. E. Google Scholar
Speck, N. M. G. Autor UNIFESP Google Scholar
Ribalta, J. C. Autor UNIFESP Google Scholar
Abstract The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec (R) Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 +/- 15.89 years) and parity ranged from 1-11 (5.92 +/- 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant

it was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotype

however, more studies are necessary to further our understanding of this restricted group.
Keywords P16 Protein
P16 Expression
Human Papillomavirus
GenotypeCervical Intraepithelial Neoplasia
Human-Papillomavirus Genotypes
Language English
Sponsor Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) grant
Date 2016
Published in Genetics And Molecular Research. Ribeirao preto, v. 15, n. 3, p. UNSP 15036840, 2016.
ISSN 1676-5680 (Sherpa/Romeo, impact factor)
Publisher Univ Fed Sao Carlos, Dept Engenharia Materials
Extent UNSP 15036840
Origin http://dx.doi.org/10.4238/gmr.15036840
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000384881100006
URI http://repositorio.unifesp.br/handle/11600/49389

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