Early ifn-gamma production together with decreased expression of tlr3 and tlr9 characterizes eae development conditional on the presence of myelin

Early ifn-gamma production together with decreased expression of tlr3 and tlr9 characterizes eae development conditional on the presence of myelin

Author Evangelista, Marcilene Gomes Google Scholar
Ribeiro De Castro, Sandra Bertelli Google Scholar
De Souza Alves, Caio Cesar Google Scholar
Dias, Alyria Teixeira Google Scholar
De Souza, Viano Wyallison Google Scholar
Dos Reis, Livia Bittencourt Google Scholar
Da Silva, Luan Cristian Google Scholar
Marques Nogueira Castanon, Maria Christina Google Scholar
Farias, Rogerio Estevam Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Ferreira, Ana Paula Google Scholar
Abstract Experimental autoimmune encephalomyelitis (EAE) is a model for the study of multiple sclerosis, which is an inflammatory and demyelinating disease of the central nervous system (CNS). Despite increased efforts to elucidate the function of toll-like receptors (TLRs) in autoimmune diseases of the CNS, the relative contribution of other factors, including the immunomodulatory properties of TLR signaling, role of the innate response and the presence or absence of myelin peptides remain unclear. The aim was to evaluate TLR expression in the CNS during EAE development by investigating the expression of TLRs in the initial phase of EAE and establishing correlations with the modulation of inflammatory factors. Mice were subcutaneously immunized at the tail base with 100 mg of myelin oligodendrocyte glycoprotein peptide (MOG(35-55)), emulsified in complete Freund's adjuvant (CFA) supplemented with 400 mg of attenuated Mycobacterium tuberculosis H37RA. Pertussis toxin (300 ng per animal) was intraperitoneally injected on the day of immunization and 48 h later. Another group (MOG(-)) received an equal emulsion of CFA and M. tuberculosis, without MOG(35-55), and the same protocol of Pertussis toxin. The immunized mice presented signs of disease with increased IFN-gamma production and presence of NK cells on Day 2 postimmunization and reduced the expression of TLR-3 and TLR-9. In the spinal cord, CCL5 and CCL20 were higher in EAE. This study establishes a correlation between TLR-3 and TLR-9 expression with the development of EAE. In addition, evidence of a role for the myelin peptide in targeting the innate inflammatory response to the CNS is presented.
Keywords Eae
Multiple Sclerosis
Toll-LikeExperimental Autoimmune Encephalomyelitis
Toll-Like Receptors
Dendritic Cells
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CNPq: 481459/2009-0
CNPq: 303369/2009-4
CNPq: 306575/2012-4
CNPq: 470768/2013-4
FAPEMIG: 02236/10
FAPEMIG: PPM 0216/10
FAPEMIG: PPM 00269-14
CAPES: PNPD-2882/2011
Date 2016
Published in Autoimmunity. Abingdon, v. 49, n. 4, p. 258-267, 2016.
ISSN 0891-6934 (Sherpa/Romeo, impact factor)
Publisher Hospital Clinicas, Univ Sao Paulo
Extent 258-267
Origin https://doi.org/10.3109/08916934.2016.1141898
Access rights Closed access
Type Article
Web of Science ID WOS:000385573300007
URI http://repositorio.unifesp.br/handle/11600/49376

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