Protease inhibitors extracted from caesalpinia echinata lam. Affect kinin release during lung inflammation

Protease inhibitors extracted from caesalpinia echinata lam. Affect kinin release during lung inflammation

Author Cruz-Silva, Ilana Autor UNIFESP Google Scholar
Nunes, Viviane Abreu Google Scholar
Gozzo, Andrezza Justino Autor UNIFESP Google Scholar
Praxedes-Garcia, Priscila Autor UNIFESP Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Shimamoto, Kazuaki Google Scholar
Araujo, Mariana Silva Autor UNIFESP Google Scholar
Abstract Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes

however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases.
Keywords Molecular-Weight Kininogen
Human Neutrophil Elastase
Bronchoalveolar Lavage
Receptor Antagonists
Asthmatic Subjects
Tissue Kallikrein
Bradykinin B-1
Language English
Sponsor Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [04/11015-0, 07/55496-0, 01/02457-0]
Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [304923/2006-0, 304719/2009-9]
Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Ministerio da Educacao Superior de Cuba (CAPES/MES), Brazil [011/06, 077/09]
Grant number FAPESP: 04/11015-0
FAPESP: 07/55496-0
FAPESP: 01/02457-0
CNPq: 304923/2006-0
CNPq: 304719/2009-9
CAPES/MES: 011/06 and 077/09
Date 2016
Published in Pulmonary Medicine. London, p. UNSP 9425807, 2016.
ISSN 2090-1836 (Sherpa/Romeo, impact factor)
Publisher Springer Heidelberg
Extent UNSP 9425807
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000390157000001

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