Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil

Antifungal susceptibility testing and genotyping characterization of cryptococcus neoformans and gattii isolates from hiv-infected patients of ribeirao preto, sao paulo, brazil

Author Figueiredo, Thais Pandini Google Scholar
de Lucas, Rosymar Coutinho Google Scholar
Cazzaniga, Rodrigo Anselmo Google Scholar
Franca, Carolina Nunes Autor UNIFESP Google Scholar
Segato, Fernando Google Scholar
Taglialegna, Rafael Google Scholar
Maffei, Cláudia Maria Leite Google Scholar
Abstract Cryptococcosis is a leading invasive fungal infection in immunocompromised patients. Considering the high prevalence and severity of these infections in immunocompromised patients attended at HC-FMRP-USP, the present research aimed to characterize the clinical isolates of Cryptococcus strains by biochemical and molecular methods and evaluate antifungal susceptibility of clinical isolates. Fifty isolates from 32 HIV-positive patients were obtained at HC-FMRP-USP. Most of the isolates (78.1%) were identified as C. neoformans, and 100% of C. neoformans and C. gattii strains were susceptible to amphotericin B, ketoconazole and fluconazole. All isolates were classified as serotype A (grubbii variety) by PCR and most of them were characterized in mating type MATa. PCR analysis of specific M13 microsatellite sequence revealed that VNI type was predominant among C. neoformans, while VGII was predominant among C. gattii. The strains did not show a significant resistance to the antifungals tested, and Canavanine-Glycine-Bromthymol Blue Agar (CGB) proved to be a reliable test presenting a good correlation with the molecular characterization. C. neoformans isolated from disseminated infections in the same patient showed molecular identity when different anatomical sites were compared

besides, the studied strains did not present a significant increase in resistance to antifungal agents. In addition, the homogeneity of the molecular types and detection of the mating types suggested a low possibility of crossing among the strains.
Keywords Cryptococcus Neoformans
Serotyping
Genotyping
Fingerprinting
Antifungal Agents
Mating Type
Hiv-PatientsPolymerase-Chain-Reaction
Species Complex
Mating-Types
Serotype-A
Molecular Characterization
Var. Gattii
Strains
Virulence
Dna
Pathogenesis
Language English
Sponsor Coordination for the Improvement of Higher Education Personnel, Brazil - (CAPES - Institutional Quota)
Date 2016
Published in Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao paulo, v. 58, p. UNSP 69, 2016.
ISSN 0036-4665 (Sherpa/Romeo, impact factor)
Publisher Wiley
Extent UNSP 69
Origin http://dx.doi.org/10.1590/S1678-9946201658069
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000396241000020
SciELO ID S0036-46652016005000253 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/49254

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