Relationship between central behavioral effects and peripheral sympathetic neurotransmission functionality during acute cocaine withdrawal syndrome in adult rats

Relationship between central behavioral effects and peripheral sympathetic neurotransmission functionality during acute cocaine withdrawal syndrome in adult rats

Author Souza Bomfim, Guilherme Henrique Autor UNIFESP Google Scholar
Garcia Garcia, Antonio Google Scholar
Jurkiewicz, Aron Autor UNIFESP Google Scholar
Jurkiewicz, Neide Hyppolito Autor UNIFESP Google Scholar
Abstract Background: Acute cocaine withdrawal syndrome (ACWS) is characterized as a set of organic alterations triggered by abrupt discontinuation of chronic cocaine consumption, usually occurring at 24-40 hours after withdrawal. However, little is known about the relationship between central and peripheral sympathetic neurotransmission during ACWS. Objective and Methods: We investigated the mechanisms involved in central and peripheral sympathetic neurotransmission and how ACWS affects the sympathetic functionality. Cocaine was administered twice daily for 5 days in Wistar rats (at least 5 in each group): on the first and second day, 15 mg/kg/i.p.

third day, 20 mg/kg/i.p.

and finally in the last two days, 30 mg/kg/i.p. Subsequently, at 1, 24, 48 and 120 h after cocaine administration the following experiments were done: (i) at the central level, behavioral tests of open-field and elevated plus maze

and (ii) at the peripheral level, tests of catecholamine release, function of (2)-adrenergic receptors ((2)-ARs), imidazoline receptors (I-1,I-2-Rs), L-type voltage-gated (Ca-v1.2) Ca2+ channels and (1)-ARs. Results: During ACWS, rats showed hypolocomotion and exacerbation of anxiogenic-effects 24 h after cocaine withdrawal. Likewise, a decrease in the catecholamine release and activity of (2)-ARs/I-1,I-2-Rs at 24-48 h after cocaine withdrawal was observed. A decrease in Ca-v1.2 channels and (1)-ARs function at 48 h after cocaine withdrawal was observed. Conclusions: The relationship of central and peripheral sympathetic neurotransmission during ACWS possibly due to a failure in activation and/or inactivation of presynaptic (2)-ARs/I-1,I-2-Rs, may offer a potential target for attenuating ACWS.
Keywords Acute Cocaine Withdrawal Syndrome
Central And Peripheral Sympathetic Neurotransmission
Calcium ChannelsElevated Plus-Maze
Vas-Deferens
Nucleus-Accumbens
Dentate Gyrus
Long-Term
In-Vitro
Dopamine
Brain
Receptors
Release
Language English
Sponsor Fundacao Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES [BEX 8477/13-2]
"Fundacao de Amparo a Pesquisa do Estado de Sao Paulo" - FAPESP
Grant number CAPES: BEX 8477/13-2
Date 2016
Published in American Journal Of Drug And Alcohol Abuse. Philadelphia, v. 42, n. 1, p. 63-76, 2016.
ISSN 0095-2990 (Sherpa/Romeo, impact factor)
Publisher Cuba Editora
Extent 63-76
Origin https://doi.org/10.3109/00952990.2015.1094082
Access rights Closed access
Type Article
Web of Science ID WOS:000371803200009
URI http://repositorio.unifesp.br/handle/11600/49200

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