Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long period

Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long period

Autor Machado, Alexandre Paulo Google Scholar
Freymüller-Haapalainen, Edna Autor UNIFESP Google Scholar
Fischman, Olga Autor UNIFESP Google Scholar
Instituição Federal University of Mato Grosso Department of Basic Health Sciences
Universidade Federal de São Paulo (UNIFESP)
Resumo The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10(4) cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in one footpad, whereas in the other group, both footpads were infected. Active lesions were observed up to seven months post-infection, particularly in mice inoculated at two sites. After this period, animals were killed. Histological sections revealed characteristics bearing a strong resemblance to the human form of the disease such as tissue hyperplasia, granulomas with microabscesses and sclerotic cells. Based on this study, we identified fungal cells from old cultures capable of provoking chronic chromoblastomycosis under experimental conditions, especially when more than one site is infected.
Palavra-chave Fonsecaea pedrosoi
experimental chromoblastomycosis
sclerotic cells
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2009-01-01
Publicado em Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 15, n. 4, p. 680-695, 2009.
ISSN 1678-9199 (Sherpa/Romeo, fator de impacto)
Publicador Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Extensão 680-695
Fonte http://dx.doi.org/10.1590/S1678-91992009000400007
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000272488500006
SciELO S1678-91992009000400007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/4797

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