Purple carrot extract protects against cadmium intoxication in multiple organs of rats: Genotoxicity, oxidative stress and tissue morphology analyses

Purple carrot extract protects against cadmium intoxication in multiple organs of rats: Genotoxicity, oxidative stress and tissue morphology analyses

Author Claudio, Samuel Range| Autor UNIFESP Google Scholar
Yamamura, Hirochi Autor UNIFESP Google Scholar
Morais, Damila Rodrigues Google Scholar
Bataglion, Giovana Anceski Google Scholar
Eberlin, Marcos Nogueira Google Scholar
Peres, Rogerio Correa Autor UNIFESP Google Scholar
Fujiyama Oshima, Celina Tizuko Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Boiago Gollucke, Andrea Pittelli Autor UNIFESP Google Scholar
Abstract The aim of this study was to investigate if purple carrot extract is able to protect against the noxious activities induced by cadmium exposure in multiple organs of rats. For this purpose, histopathological analysis, genotoxicity and oxidative status were investigated in this setting. A total of twenty Wistar rats weighing 250 g on the average, and 8 weeks age were distributed into four groups (n=5), as follows: Control group (non-treated group, CTRL) Cadmium group (Cd) and Purple carrot extract groups at 400 mg/L or 800 mg/L. Histopathological analysis revealed that liver from animals treated with purple carrot extract improved tissue degeneration induced by cadmium intoxication. Genetic damage was reduced in blood and hepatocytes as depicted by comet and micronucleus assays in animals treated with purple carrot extract. SOD-CuZn and cytocrome C gene expression increased in groups treated with purple carrot extract. Purple carrot extract also reduced the 80HdG levels in liver cells when compared to cadmium group. Taken together, our results demonstrate that purple carrot extract is able to protect against cadmium intoxication by means of reducing tissue regeneration, genotoxicity and oxidative stress in multiple organs of Wistar rats.
Keywords Purple carrot
Cadmium
Rat
Genotoxicity
Oxidative stressGrape Juice Concentrate
Dna-Damage
Cytochrome-C
Wistar Rats
Blood-Cells
In-Vivo
Liver
Anthocyanins
Apoptosis
Exposure
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2016
Published in Journal Of Trace Elements In Medicine And Biology. Jena, v. 33, p. 37-47, 2016.
ISSN 0946-672X (Sherpa/Romeo, impact factor)
Publisher Elsevier Gmbh, Urban & Fischer Verlag
Extent 37-47
Origin http://dx.doi.org/10.1016/j.jtemb.2015.08.006
Access rights Closed access
Type Article
Web of Science ID WOS:000367110100007
URI http://repositorio.unifesp.br/handle/11600/46082

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