Effects of low-intensity non-coherent light therapy on the inflammatory process in the calcaneal tendon of ovariectomized rats

Effects of low-intensity non-coherent light therapy on the inflammatory process in the calcaneal tendon of ovariectomized rats

Author Helrigle, Carla Google Scholar
Camilo de Carvalho, Paulo deTarso Google Scholar
Casalechi, Heliodora Leao Google Scholar
Pinto Leal-Junior, Ernesto Cesar Google Scholar
Cardoso Fernandes, Guilherme Henrique Google Scholar
Helrigel, Panmera Almeida Google Scholar
Rabelo, Rogerio Leao Google Scholar
Aleixo-Junior, Ivo de Oliveira Google Scholar
Aimbire, Flavio Autor UNIFESP Google Scholar
Albertini, Regiane Google Scholar
Abstract The aim of this experimental study was to investigate the effects of low-intensity light-emitting diode (LED) phototherapy on the inflammatory process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of the inflammatory mediators interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-alpha). Thirty-five female Wistar rats were divided into 4 groups: 3 groups of OVX rats totaling 30 rats (untreated OVX rats [OVX injury group], treated OVX rats [OVX LED group], and control OVX rats subgroups existed based on the sampling times, which were 3, 7, and 14 days) and 1 group of non-OVX rats (not OVX n = 5). Tendon injury was induced by trauma using a 208-g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 h after tendonitis with LED therapy and every 48 h thereafter until euthanasia (at 3, 7, or 14 days). The tendons were dissected and stored in liquid nitrogen at -196 A degrees C, thawed only at the time of immunoenzymatic testing (ELISA). Groups treated with LED showed a decrease in the number of pro-inflammatory cells, IL-6, and TNF-alpha (p < 0.05), and an increase in IL-10 (p < 0.05) when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED treatment using the parameters and wavelength of 945 nm in the time periods studied reduced the release of IL-6 and TNF-alpha and increased the release of IL-10, thereby improving the inflammatory response in OVX rats.
Keywords Inflammation
Ovariectomy
Low-intensity LED
Photobiomodulation therapy
Calcaneal tendon injuryLevel Laser Therapy
Collagenase-Induced Tendinitis
Emitting Diode Therapy
Achilles-Tendon
Experimental-Model
Estrogen Deficiency
Injury
Repair
Nm
Phototherapy
Language English
Sponsor Multi Radiance Medical (Solon, OH, USA)
Date 2016
Published in Lasers In Medical Science. London, v. 31, n. 1, p. 33-40, 2016.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer London Ltd
Extent 33-40
Origin http://dx.doi.org/10.1007/s10103-015-1821-7
Access rights Closed access
Type Article
Web of Science ID WOS:000367695100006
URI http://repositorio.unifesp.br/handle/11600/46067

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