Molecular features of nonionic detergents involved in the binding kinetics and solubilization efficiency, as studied in model (Langmuir films) and biological (Erythrocytes) membranes

Molecular features of nonionic detergents involved in the binding kinetics and solubilization efficiency, as studied in model (Langmuir films) and biological (Erythrocytes) membranes

Author Casadei, Bruna Renata Autor UNIFESP Google Scholar
Domingues, Cleyton Crepaldi Google Scholar
Clop, Eduardo M. Google Scholar
Couto, Veronica Muniz Google Scholar
Perillo, Maria Angelica Google Scholar
Paula, Eneida de Google Scholar
Abstract The effect of the nonionic detergents Brij-98 and Brij-58 over human erythrocytes was studied through quantitative hemolysis and in Langmuir films. Hemolytic tests revealed that Brijs are stronger membrane solubilizers than Triton X-100 (TX-100), with effective detergent/lipid ratios of 0.18 and 0.37 for Brij-98 and Brij-58, respectively. Experiments with Langmuir films provided significant information on the kinetics and thermodynamics of detergent-membrane interaction. The adsorption (k(a)) and desorption (k(d)) rate constants of Brijs were lower than those of TX-100. In the case of k(a), that is probably due to their larger hydrophilic head (with twice (20) the oxyethylene units of TX-100). As for the thermodynamic binding constant, the linear and longer hydrophobic acyl chains of Brijs favor their stabilization in-between the lipids, through London van der Waals forces. Consequently, K-b,K-m values of Brij-98 (12,500 M-1) and Brij-58 (19,300 M-1) resulted higher than TX-100 (7500 M-1), in agreement with results from the hemolytic tests. Furthermore, Brij-58 binds with higher affinity than Brij-98 to bilayers and monolayers, despite its shorter (palmitic) hydrocarbon chain, showing that unsaturation restrains the detergent insertion into these environments. Our results provide significant information about the mechanism of interaction between Brijs and membranes, supporting their distinct solubilization effect. (C) 2018 Elsevier B.V. All rights reserved.
Keywords Erythrocyte membranes
Solubilization
Monolayers
Nonionic detergents
Brij
Language English
Sponsor Capes (Brazil)
Mincyt
SeCyT-Universidad Nacional de Cordoba
CONICET
Foncyt (Argentina)
CNPq
CAPES
Grant number CNPq: 479993/2011-4
CNPq: 308621/2013-1
Date 2018
Published in Colloids And Surfaces B-Biointerfaces. Amsterdam, v. 166, p. 152-160, 2018.
ISSN 0927-7765 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 152-160
Origin http://dx.doi.org/10.1016/j.colsurfb.2018.03.012
Access rights Closed access
Type Article
Web of Science ID WOS:000432505900019
URI http://repositorio.unifesp.br/handle/11600/46000

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