Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

Interrelationship between chromosome 8 aneuploidy, C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

Author Calcagno, Danielle Queiroz Autor UNIFESP Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Seabra, Aline Damaceno Google Scholar
Khayat, Andre Salim Google Scholar
Chen, Elizabeth Suchi Autor UNIFESP Google Scholar
Demachki, Samia Google Scholar
Assumpção, Paulo Pimentel Autor UNIFESP Google Scholar
Girao Faria, Mario Henrique Google Scholar
Rabenhorst, Silvia Helena Barem Google Scholar
Ferreira, Márcia Valéria Pitombeira Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Burbano, Rommel Rodriguez Autor UNIFESP Google Scholar
Institution Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Ceara
Abstract AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors.METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed.RESULTS: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm.CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways. (C) 2006 The WJG Press. All rights reserved.
Keywords chromosome 8 aneuploidy
C-MYC amplification
immunostaining
gastric adenocarcinoma
Language English
Date 2006-10-14
Published in World Journal Of Gastroenterology. Beijing: W J G Press, v. 12, n. 38, p. 6207-6211, 2006.
ISSN 1007-9327 (Sherpa/Romeo, impact factor)
Publisher W J G Press
Extent 6207-6211
Origin http://dx.doi.org/10.3748/WJG.v12.i38.6207
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000241371400021
URI http://repositorio.unifesp.br/11600/45742

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