Assembly of high molecular weight kininogen and activation of prekallikrein on cell matrix

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dc.contributor.author Motta, G.
dc.contributor.author Shariat-Madar, Z.
dc.contributor.author Mahdi, F.
dc.contributor.author Sampaio, Claudio AM [UNIFESP]
dc.contributor.author Schmaier, A. H.
dc.date.accessioned 2018-06-18T12:12:16Z
dc.date.available 2018-06-18T12:12:16Z
dc.date.issued 2001-09-01
dc.identifier.citation Thrombosis And Haemostasis. Stuttgart: F K Schattauer Verlag Gmbh, v. 86, n. 3, p. 840-847, 2001.
dc.identifier.issn 0340-6245
dc.identifier.uri http://repositorio.unifesp.br/11600/45660
dc.description.abstract Investigations determined if extracellular matrix of endothelial cells (EC) is a platform for HK assembly and PK activation. In buffers containing bovine serum albumin, biotin-RK binding to ECV304 cells or their matrix requires greater than or equal to 50 muM added Zn2+. Ortho-phenanthroline or a HK domain 5 peptide blocks HK binding, Binding to umbilical vein EC or matrix, but not ECV304 cells or matrix, is mediated by cytokeratin 1. Biotin-HK binds to ECV304 cells or matrix with a K-d of 15.8 or 9.0 nM and a B-max of 2.6 X 10(7) or 21.4 X 10(7) sites/cell, respectively. PK activation un ECV304 cells or matrix is blocked by antipain or SBTI and corn trypsin inhibitor partially inhibits kallikrein formation. PK activation occurs on ECV304 cells or matrix prepared without serum or in human factor XII deficient serum. indicating that the PK activator is not factor XIIa. EC matrix promotes plasminogen activation after the assembly of HK, PK and pro-urokinase. These studies indicate that matrix of various EC has the ability to assemble HK allowing for PK activation and subsequent activities. en
dc.format.extent 840-847
dc.language.iso eng
dc.publisher F K Schattauer Verlag Gmbh
dc.relation.ispartof Thrombosis And Haemostasis
dc.rights Acesso restrito
dc.subject HMW kininogen en
dc.subject prekallikrein en
dc.subject endothelial cells en
dc.subject extracellular matrix en
dc.subject pro-urokinase en
dc.subject plasminogen en
dc.title Assembly of high molecular weight kininogen and activation of prekallikrein on cell matrix en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Michigan
dc.description.affiliation UNIFESP, EPM, Dept Bioquim, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliation Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Bioquim, BR-04044020 Sao Paulo, SP, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000170992600019



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