A new factor Xa inhibitor (lefaxin) from the Haementeria depressa leech

A new factor Xa inhibitor (lefaxin) from the Haementeria depressa leech

Author Faria, Fernanda Google Scholar
Kelen, Eva Maria A Google Scholar
Sampaio, Claudio AM Autor UNIFESP Google Scholar
Bon, Cassian Google Scholar
Duval, Nathalie Google Scholar
Chudzinski-Tavassi, Ana Marisa Google Scholar
Institution Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Inst Pasteur
Abstract The salivary complex of the leech Haementeria depressa produces potent anticoagulant components. Among them, a protein named lefaxin inhibits factor Xa (FXa). Lefaxin was purified to homogeneity from dissected salivary complexes by gel filtration in Sephadex G-150 followed by two ion exchange chromatography steps in Mono-Q. Inhibition of FXa by lefaxin was demonstrated by the inhibition of its amidolytic activity, measured with chromogenic substrate S-2765 (apparent K-1 of 1 nM), and of its ability to inhibit thrombin generation in the prothrombinase complex (EC50 of 40 nM). Lefaxin has a molecular weight of 30 kDa and an isoelectric point of 5.7. It is made of a poly peptide chain whose N-terminal sequence shows no similarity with that of other FXa inhibitors (antistasin and ghilianten) isolated from leech saliva, On the other hand, the N-terminal sequence of lefaxin presents significant sequence similarity with nitric oxide carrier proteins myohemerythrin from the annelid Nereis diversicolor and prolixin S from the tick Rhodnius prolixus. Interestingly, prolixin S also proved to be an anticoagulant protein acting on FXa.
Keywords leeches
FXa inhibitor
Haementeria depressa
Language English
Date 1999-11-01
Published in Thrombosis And Haemostasis. Stuttgart: F K Schattauer Verlag Gmbh, v. 82, n. 5, p. 1469-1473, 1999.
ISSN 0340-6245 (Sherpa/Romeo, impact factor)
Publisher F K Schattauer Verlag Gmbh
Extent 1469-1473
Access rights Closed access
Type Article
Web of Science ID WOS:000083805100020
URI http://repositorio.unifesp.br/11600/45658

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