Fibrino(geno)lytic properties of purified hementerin, a metalloproteinase from the leech Haementeria depressa

Fibrino(geno)lytic properties of purified hementerin, a metalloproteinase from the leech Haementeria depressa

Author Chudzinski-Tavassi, Ana Maria Google Scholar
Kelen, Eva Maria A Google Scholar
Paula Rosa, Ana Paula de Google Scholar
Loyau, Stephane Google Scholar
Sampaio, Claudio Augusto Machado Autor UNIFESP Google Scholar
Bon, Cassian Google Scholar
Angles-Cano, Eduardo Google Scholar
Institution Inst Butantan
Hop Bicetre
Universidade Federal de São Paulo (UNIFESP)
Inst Pasteur
Abstract The fibrino(geno)lytic protein designated hementerin contained in crude extracts of the salivary complex of Haementeria depressa leeches was purified to apparent homogeneity by gel filtration, ion exchange chromatography and preparative SDS-PAGE. It is a single-chain 80 kDa, PhMeSO2F-resistant, calcium-dependent, metalloproteinase, which specifically degrades fibrin(ogen) through a plasminogen-independent pathway. The amino terminal sequence of 8 residues shows 80% similarity with hementin, another fibrino(geno)lytic protein purified from Haementeria ghilianii leeches. However, their activities differ somewhat in terms of kinetics and with regard to the structure of the fibrin(ogen) fragments they may produce. Cleavage by hementerin of fibrinogen A alpha, gamma and B beta chains, in that order, produces 270 kDa to 67 kDa fragments which differ from those produced by plasmin. Hementerin was also able to degrade cross-linked fibrin although at a lower rate as compared to fibrinogen. In conclusion, hementerin is a plasminogen-independent fibrino(geno)lytic metalloproteinase that degrades fibrinogen faster than fibrin, prevents blood coagulation and destroys fibrin clots in vitro.
Language English
Date 1998-07-01
Published in Thrombosis And Haemostasis. Stuttgart: F K Schattauer Verlag Gmbh, v. 80, n. 1, p. 155-160, 1998.
ISSN 0340-6245 (Sherpa/Romeo, impact factor)
Publisher F K Schattauer Verlag Gmbh
Extent 155-160
Access rights Closed access
Type Article
Web of Science ID WOS:000074787600026

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