MINIMUM FRAGMENTS OF THE HEPARIN MOLECULE ABLE TO PRODUCE THE ACCUMULATION AND CHANGE OF THE SULFATION PATTERN OF AN ANTITHROMBOTIC HEPARAN-SULFATE FROM ENDOTHELIAL-CELLS

MINIMUM FRAGMENTS OF THE HEPARIN MOLECULE ABLE TO PRODUCE THE ACCUMULATION AND CHANGE OF THE SULFATION PATTERN OF AN ANTITHROMBOTIC HEPARAN-SULFATE FROM ENDOTHELIAL-CELLS

Author Pinhal, MAS Google Scholar
Santos, IAN Google Scholar
Silva, I. F. Google Scholar
Dietrich, Carl Peter Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Heparin and low molecular weight heparins stimulate two to three fold the accumulation of an antithrombotic heparan sulfate secreted by endothelial cells in culture. This led us to search for the minimum structural requirements of the heparin molecule able to elicit the enhancement of the heparan sulfate. Fragments were prepared from heparin by degradation with bacterial heparinase and heparitinases. A heparin pentasulfated tetrasaccharide was shown to be the minimum structural sequence able to enhance two to three fold the secretion of heparan sulfate by endothelial cells. The stimulation is specific for the endothelial cell, is concentration dependent and the effect is already noticed after one hour of exposure of the cells to heparin and the tetrasaccharide. Degradation of the [S-35]-heparan sulfate synthesized in the presence of heparin or the tetrasaccharide has shown a higher degree of sulfation of its iduronic acid residues.
Language English
Date 1995-10-01
Published in Thrombosis And Haemostasis. Stuttgart: F K Schattauer Verlag Gmbh, v. 74, n. 4, p. 1169-1174, 1995.
ISSN 0340-6245 (Sherpa/Romeo, impact factor)
Publisher F K Schattauer Verlag Gmbh
Extent 1169-1174
Access rights Closed access
Type Article
Web of Science ID WOS:A1995TA95100031
URI http://repositorio.unifesp.br/11600/45654

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